Thesis (PhD)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Antimicrobial fibers are very useful in various fields such as air and water purification,
wound dressings and protective bandages, where sterile environments are essential. The nonwoven
nanofiber mats or membranes are able to filter out microorganisms and potentially
kill several threatening pathogenic bacteria. In this thesis, a variety of styrene-maleimide
copolymer derivatives were prepared based on the modification of poly(styrene-co-maleic
anhydride with various primary amine compounds.
All prepared copolymer derivatives were electrospun to nanofiber mats using the needle
electrospinning technique. For the characterization, differential scanning calorimetry (DSC)
and thermogravimetric analysis (TGA) were used to study the thermal properties of the
electrospun fiber mats. Scanning electron microscopy (SEM) was carried out to observe fiber
dimensions and morphology.
The antibacterial activity of electrospun fiber mats was evaluated against different bacteria
including Staphylococcus aureus (Gram-positive), Escherichia coli and Pseudomonas
aeruginosa (Gram-negative). The evaluation study utilized different tools to test for
antibacterial activity and mode of cell death, including bioluminescent imaging, fluorescence
imaging and the viable cell counting method. Excellent antimicrobial activity was obtained
against the different strains especially against Staphylococcus aureus. Fiber mats containing
tertiary amino groups, phenol or quaternary ammonium groups had the strongest
antimicrobial properties. / AFRIKAANSE OPSOMMING: Antimikrobiese vesels is baie nuttig in verskeie toepassingsgebiede, soos lug- en
watersuiwering, wondbedekkings en beskermende verbande, waar ‘n steriele omgewing
noodsaaklik is. Die ongeweefde nanovesel matte of membrane is in staat om mikroorganismes
te verwyder deur filtrasie, maar kan ook verskeie patogeniese bakterieë
doodmaak. In hierdie proefskrif is ‘n verskeidenheid stireen-maleimied kopolimeer
afgeleides gesintetiseer, gebaseer op die modifikasie van poli(stireen-ko-maleïne anhidried)
met verskeie primêre amien verbindings.
Nanovesel matte van al die gesintetiseerde kopolimeer afgeleides is gemaak deur gebruik te
maak van die naald-elektrospin tegniek. Die termiese eienskappe van hierdie nanovesel matte
is bestudeer deur gebruik te maak van differensiële skandeer kalorimetrie (DSK) en
termogravitasie analiese (TGA) as karakteriseringsmetodes. Die vesel dimensies en
morfologie is bestudeer deur skandeer elektronmikroskopie as karakteriseringsmetode te
gebruik.
Die antibakteriële aktiwiteit van die gespinde vesel matte is geëvalueer teen verskillende
bakterieë, naamlik Staphylococcus aureus (Gram-positief), Escherichia coli en Pseudomonas
aeruginosa (Gram-negatief). Die evalueringstudie het verskillende instrumente gebruik om
vir antibakteriële aktiwiteit en meganisme van seldood te toets, insluitend bioluminiserings
beelding, fluoressensie beelding en die lewensvatbare sel tellingsmetode. Uitstekende
antimikrobiese aktiwiteit is verkry teen die verskillende rasse, veral teen Staphylococcus
aureus. Vesel matte met tersiêre aminogroepe, fenol of kwaternêre ammoniumgroepe het die
sterkste antimikrobiese eienskappe gehad.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:sun/oai:scholar.sun.ac.za:10019.1/20160 |
| Date | 03 1900 |
| Creators | Bshena, Osama E. S. |
| Contributors | Klumperman, Bert, Stellenbosch University. Faculty of Science. Dept. of Chemistry and Polymer Science. |
| Publisher | Stellenbosch : Stellenbosch University |
| Source Sets | South African National ETD Portal |
| Language | en_ZA |
| Detected Language | Unknown |
| Type | Thesis |
| Rights | Stellenbosch University |
Page generated in 0.0022 seconds