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Previous issue date: 2010-08-19 / Regarding the measurement of tear production, the technique of reading tear with a cotton soaked in a pH indicator solution has proven to be advantageous compared to Schirmer Tears Test in many studies due to the smaller width of the test material and time for attaining this , which leads to less reflex tear production and a closer reading of basal tear production or real. This experiment aimed to develop a quantitative measurement equipment in tear production similar to the ZoneQuick? Menicon and improve it, establish a pattern of reading to those and evaluate their advantages and disadvantages. For that, we selected 50 beagle dogs, male and female, aged 1-8 years from the Canil do Laboratorio de Quimioterapia Experimental Parasitol?gica of UFRRJ, and produced two tests with Cotton Thread with Phenol Red and Bromocresol Green. Afterwards was made the reading standization of those three tests compared to the Schirmer tear test, getting as normal range of reading for the tests with Cotton Thread with Phenol Red 13.6 to 22.7 mm/15seg., the ZoneQuick? Menicon of between 22, 7 and 37.8 mm/15seg. and tests with Cotton Thread with the Bromocresol Green 13.7 to 22.8 mm/15seg.. Among those three tests, the ZoneQuick? showed the smallest shift in color of the indicator and the largest Cotton Thread with the Bromocresol Green which along with the smaller standard error presented by this facilitates its reading and reduces the chance of false positive or negative. In a comparison between the Schirmer Tears Test , Cotton Thread with the Bromocresol Green tests and Cotton Thread with Phenol Red latter, generated less reflex tear production, with a reading closer to the real. / Em rela??o ? mensura??o da produ??o lacrimal, a t?cnica de leitura lacrimal com o fio de algod?o embebido em uma solu??o indicadora de pH vem se mostrando vantajosa em rela??o ao Teste Lacrimal de Schirmer I (TLS I) em diversos estudos, devido ? menor largura do material de teste e tempo de realiza??o deste, o que leva a uma menor produ??o lacrimal reflexa e uma leitura mais pr?xima da produ??o lacrimal basal ou real. Este experimento teve como objetivos, testar diferentes tipos de fios e avaliar qual o mais indicado a realiza??o dos testes, desenvolver um material de medi??o quantitativo da produ??o lacrimal de fio impregnado com vermelho fenol, testar outro indicador de pH, estabelecer um padr?o de leitura para estes e o ZoneQuick? da Menicon e compar?-los ao TLS I avaliando suas vantagens e desvantagens. Para isto, foram selecionados 50 c?es da ra?a Beagle, machos e f?meas, com idade variando de 1 a 8 anos do Canil do Laborat?rio de Quimioterapia Experimental Parasitol?gica da UFRRJ, e produzidos dois testes com Fio de Algod?o impregnados com Vermelho Fenol ou Verde de Bromocresol. Em seguida foi feita a padroniza??o da leitura dos tr?s testes em compara??o ao TLS I, obtendo-se como faixa normal de leitura para o Teste de Fio de Algod?o com Vermelho Fenol de 13,6 a 22,7 mm/15seg., o ZoneQuick? da Menicon entre 22,7 e 37,8mm/15seg. e o Teste de Fio de Algod?o com Verde de Bromocresol de 13,7 a 22,8 mm/15 seg.. Dentre os tr?s testes, o ZoneQuick? apresentou a menor viragem na cor do indicador e o Teste de Fio de Algod?o com Verde de Bromocresol a maior o que, juntamente com o menor erro padr?o por este apresentado, facilita sua leitura e reduz a chance de falso positivo ou negativo. Na etapa de compara??o entre os TLS I, Fio de Algod?o com Verde de Bromocresol e Fio de Algod?o com Vermelho Fenol este ?ltimo gerou menor produ??o lacrimal reflexa, com uma leitura mais pr?xima da real.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2455 |
| Date | 19 August 2010 |
| Creators | Silva, Leandro Nogueira e |
| Contributors | Pereira, Jo?o Telhado |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Medicina Veterin?ria (Patologia e Ci?ncias Cl?nicas), UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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