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Previous issue date: 2017-02-23 / O laser de baixa intensidade (LBI) tem apresentado resultados positivos na
prolifera??o de diversos tipos celulares in vitro. A primeira parte do trabalho
avaliou o efeito do LBI na prolifera??o e viabilidade de c?lulas-tronco da polpa
de dentes dec?duos humanos esfoliados (SHED). As c?lulas foram irradiadas
ou n?o (controle) com um laser diodo InGaAlP (660 nm, 30 mW, modo de a??o
cont?nuo) usando duas diferentes doses (0,5 J/cm2 por 16 segundos e 1,0
J/cm2 por 33 segundos) e os tr?s grupos foram estudados nos intervalos de 0,
24, 48 e 72 h. Foi observado que a dose de 1,0 J/cm2 promoveu um aumento
na prolifera??o celular nos intervalos de 48 e 72 h quando comparada ao grupo
controle e ? dose de 0,5 J/cm2 e a viabilidade celular n?o foi afetada pela
irradia??o ao longo do experimento. Em conjunto, os dados mostraram que os
par?metros do LBI utilizados (660 nm, 30 mW, 1,0 J/cm2) promoveram
prolifera??o das SHEDs e mantiveram a sua viabilidade. A partir destes
resultados favor?veis, a segunda parte do trabalho avaliou o efeito do LBI com
os mesmos par?metros na prolifera??o de SHEDs cultivadas sobre arcabou?os
nanofibrosos de PLA confeccionados pela t?cnica de eletrofia??o. Este
procedimento permite a obten??o de arcabou?os tridimensionais que simulam
a matriz extracelular a partir de um biomaterial com propriedades f?sicas
favor?veis e baixo custo. Tr?s grupos experimentais (G1 ? n?o irradiado; G2 ?
irradiado com 0,5 J/cm2; G3 ? irradiado com 1,0 J/cm2) foram analisados nos
intervalos de 24, 48 e 72 h. Os resultados indicaram que o PLA n?o ? citot?xico
para as SHEDs e que os grupos submetidos ? laserterapia tiveram maior
prolifera??o celular quando comparados com o grupo controle n?o irradiado.
Com base nos achados, evidenciou-se que as nanofibras de PLA s?o
arcabou?os favor?veis para o cultivo de SHEDs e que a laserterapia estimulou
a prolifera??o quando aplicada nas c?lulas cultivadas sobre este arcabou?o.
Com base nos dados gerais obtidos, conclui-se que a laserterapia nos
par?metros avaliados apresenta efeitos bioestimulat?rios nas SHEDs
cultivadas tanto na superf?cie padr?o (pl?stico da placa de cultivo) quanto sobre
arcabou?os nanoestruturados de PLA. / The low-level laser therapy (LLLT) has been shown positive results in the in
vitro proliferation of several cell types. The first part of the study evaluated the
effect of LLLT on the proliferation and viability of stem cells from human
exfoliated deciduous teeth (SHED).Cells were irradiated or not (control) with an
InGaAlP diode laser (660 nm, 30 mW, continuous action mode) using two
different doses (0.5 J/cm2 for 16 seconds and 1.0 J/cm2 for 33 seconds) and the
three groups were analyzed at the intervals of 0, 24, 48 and 72 h.It was
observed that the dose of 1.0 J/cm2 promoted an increase in cell proliferation at
the 48 and 72 h intervals when compared to the control group and the dose of
0.5 J/cm2, and that cell viability was not affected by irradiation throughout the
experiment. Taken together, the data showed that the LLLT parameters (660
nm, 30 mW, 1.0 J/cm2) promoted proliferation of SHEDs and maintained their
viability. Based on these favorable results, the second part of the study
evaluated the effect of LLLT with the same parameters on the proliferation of
SHEDs cultured on PLA nanofibrous scaffolds obtained by electrospinning. This
procedure allows obtaining three-dimensional scaffolds that mimic the
extracellular matrix from a biomaterial with favorable physical properties and
low cost.Three experimental groups (G1 - non-irradiated; G2 - irradiated with
0.5 J/cm2; G3 - irradiated with 1.0 J/cm2) were analyzed at 24, 48 and 72 h. The
results indicated that PLA is not cytotoxic to SHEDs and that the groups
submitted to laser therapy had higher cell growth when compared to the nonirradiated
control group. Based on these findings, it was shown that PLA
nanofibers are favorable scaffolds for the cultivation of SHEDs and that laser
therapy stimulated the proliferation when applied to the cells cultured on this
scaffold. Based on the general data obtained, it is concluded that the laser
therapy in the evaluated parameters has biostimulatory effects on the
proliferation of SHEDs on both the standard surface (plastic of the culture plate)
and on nanostructured PLA scaffolds.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/23656 |
| Date | 23 February 2017 |
| Creators | Antunes, Fernanda Ginani |
| Contributors | 67269621420, http://lattes.cnpq.br/5004397230198722, Moura, Carlos Eduardo Bezerra de, 03597959431, http://lattes.cnpq.br/4717410137206021, Lucena, Eudes Euler de Souza, 01360681469, http://lattes.cnpq.br/6969519171915250, Rocha, Hugo Alexandre de Oliveira, 76111830449, http://lattes.cnpq.br/4651814546820796, Souza, Lelia Batista de, 08588724472, http://lattes.cnpq.br/6671914892609743, Barboza, Carlos Augusto Galv?o |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM PATOLOGIA ORAL, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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