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Previous issue date: 2016-02-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Apesar da r?pida expans?o nas ?ltimas d?cadas, o cultivo de macroalgas ainda n?o atende ? demanda das ind?strias por material algal. Neste sentido, ? necess?rio desenvolver protocolos que possibilitem a implanta??o de cultivos comerciais e evitem a sobre-explora??o dos bancos naturais. A propaga??o vegetativa e o cultivo sob pulsos s?o m?todos que permitem minimizar custos e produzir uma grande quantidade de prop?gulos de maneira sustent?vel. Este estudo teve como objetivo avaliar o crescimento de prop?gulos de Gracilaria birdiae cultivados sob pulsos de nutrientes. O estudo foi dividido em tr?s experimentos: (1) indoor, (2) outdoor e (3) em campo. O experimento indoor, realizado sob condi??es controladas, foi conduzido em 12 aqu?rios (10L) com ?gua do mar filtrada e 40 prop?gulos de G. birdiae (~ 0,5 g/L). Os prop?gulos foram submetidos a tr?s tratamentos, em triplicata, com pulsos de fertiliza??o: (T1) efluente de viveiro de camar?o; (T2) fertilizante para aquariofilia (Mbreda?); (T3) fertilizante extra?do de Ascophyllum nodosum (Acadian?). Uma s?rie de tr?s aqu?rios (sem pulsos de fertiliza??o) foi utilizada como controle. O experimento outdoor, onde os prop?gulos estiveram sujeitos ?s varia??es ambientais, foi conduzido em 12 aqu?rios (30L), com densidade de aproximadamente 1 g/L de biomassa, e submetidos aos mesmos tratamentos descritos acima. O experimento em campo consistiu no cultivo dos prop?gulos dos experimentos anteriores (indoor e outdoor) no mar e em uma fazenda de camar?o org?nico. Os valores positivos de biomassa e da taxa de crescimento em todos os experimentos evidenciam a viabilidade de produzir biomassa de Gracilaria birdiae da propaga??o in vitro at? o cultivo em campo. Os prop?gulos cultivados no tratamento T2 e no T3 apresentaram os maiores valores de biomassa e de taxa de crescimento, assim como o maior conte?do de nitrog?nio. Isto indica que os dois fertilizantes possuem os nutrientes necess?rios e em propor??es balanceadas para o crescimento de G. birdiae. Os prop?gulos cultivados na fazenda de camar?o org?nico obtiveram valores de biomassa e taxas de crescimento menores do que aqueles cultivados no mar devido ? maior varia??o da temperatura e da salinidade. Nestas condi??es estressantes, os prop?gulos do T3 demonstraram desempenho superior em virtude dos compostos bioativos presentes no extrato de A. nodosum, os quais aumentam a resist?ncia ao estresse abi?tico. De acordo com os resultados obtidos, os prop?gulos apresentaram maior crescimento quando cultivados sob pulsos de fertilizantes e o protocolo desenvolvido pode ser aplicado para produ??o de estoques de prop?gulos. / Despite the rapid expansion in recent decades, seaweed cultivation still does not meet the demand of industries for algal materials. In this sense, it is necessary to develop protocols that enable the deployment of commercial cultivations and avoid overexploitation of natural beds. Vegetative propagation and cultivation under pulses are methods that minimize costs and produce a great amount of propagules in a sustainable way. This study aimed to evaluate the growth of Gracilaria birdiae propagules cultivated under nutrient pulses. The study was divided into three experiments: (1) indoor, (2) outdoor and (3) in the field. The indoor experiment, performed under controlled conditions, was conducted in 12 aquaria (10 L) with filtered seawater and 40 propagules of G. birdiae (~ 0.5 g / L). The propagules were submitted to three treatments, in triplicate, with fertilization pulses: (T1) shrimp-pond effluent; (T2) fertilizer for aquarium (Mbreda?); (T3) fertilizer extracted from Ascophyllum nodosum (Acadian?). A series of three aquaria (without fertilization pulses) was used as control. The outdoor experiment, where the propagules have been exposed to environmental variations, was conducted in 12 aquaria (30 L), with a density of about 1 g/L of biomass, and submitted to the same treatments described above. The field experiment consisted in the cultivation of the propagules from the previous experiments (indoor and outdoor) in the sea and in an organic shrimp farm. Propagules that were cultivated in treatments T2 and T3 showed the highest values of biomass and growth rate throughout the experiments as well as higher content of nitrogen. This indicates that both fertilizers have the necessary nutrients and in balanced proportions for growth of G. birdiae. The propagules that were cultivated in the organic shrimp farm obtained lower biomass values and growth rates than those cultivated in the sea because of the greater variation of temperature and salinity. Under these stressing conditions, propagules of T3 performed much better due to the bioactive compounds in the A. nodosum extract, which increase the resistance to abiotic stress. Positive values of biomass and growth rate in all experiments show the viability of producing G. birdiae biomass from in vitro propagation to cultivation on field. According to the obtained results, the propagules had better performance when cultivated under pulses of fertilizers and the developed protocol may be applied to the production of propagule stocks.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21337 |
| Date | 29 February 2016 |
| Creators | Fernandes, Felipe de Oliveira |
| Contributors | 18238050430, http://lattes.cnpq.br/2017823516741199, Araujo, Andre Luis Calado, 37695045268, http://lattes.cnpq.br/7133712883742750, Carneiro, Marcella Araujo do Amaral, 01184748470, http://lattes.cnpq.br/2397478558863939, N?brega, Marcelo Francisco de, 14839619824, http://lattes.cnpq.br/6194914254799842, Soriano, Eliane Marinho |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM ECOLOGIA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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