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Previous issue date: 2003-12-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Protein and caloric malnutrition has been considered one of the most concerned endemic diseases in Brazil and in the world. It has been known that depletion or reduction of proteins as far as meals are concerned can steer irreversible damages upon several organic systems. This study had as aim evaluate the effects the low-protein diet had over the formation and composition of the teeth components. 18 females and 6 males were used for the experiment. 12 from the 18 females had undertaken the low-protein diet (DH) for 03 weeks and the other 6, which remained, and those males had undertaken a controlled diet (DC) for the same period. All animals had the diets during their mating, pregnancy and lactation cycle. As soon as the offsprings had been born, 10 young males and females of each group faced a disease hood analysis to check the teeth germs of their lower fore teeth. The rest of the group had their lactation cycle normally 60 days. Then they were put to death and had their lower fore teeth removed both to be analyzed through a scanning electronic microscopy (SEM) of the structure alterations and to have their calcium checked by an atomic absorption of the phosphorus vanadate-molibdate method and by other minerals EDX method. The animals livers were removed to have their hepatic proteins analyzed as well. The histopatologic study showed that at first day of birth, all animals had their lower fore teeth come out. It was verified that 90% of the animals teeth were in an apposition and calcification period and it was possible to observe the dentin formation from 60% of the 90% already mentioned. Through the SEM method it could be realized that 90% of the animals of the DH group had their lower fore teeth easily broken and no definite shape. In this same group itself, it was also observed long micro fissures 369,66 nm ? 3,45 while the DC group had fissures of 174 nm ? 5,72. Now regarding the calcium and phosphorus concentration, it could be noticed that there was a great reduction of these components and other minerals in the DH group. Almost all minerals, except for the Cl and K, presented higher levels in the DC group enamel.The reduction of the protein input greatly influenced the offsprings? weight and height. However the hepatic proteins had no important difference between the groups what can make one believe that those animals suffered from protein malnutrition of marasmic kind / A desnutri??o prot?ico-cal?rica vem sendo classificada como uma das mais preocupantes endemias n?o s? no Brasil, mas no mundo. Sabe-se que a deple??o ou a diminui??o do aporte de prote?nas das refei??es pode provocar danos irrevers?veis a v?rios sistemas org?nicos. O objetivo do presente estudo foi avaliar o efeito da dieta hipoproteica sobre a forma??o e composi??o dos elementos dent?rios. Foram utilizados 18 f?meas e 6 machos para a realiza??o do experimento. Das 18 f?meas, 12 foram submetidas ? dieta hipoprot?ica (DH) durante 03 semanas e as outras 6 e os machos foram mantidos durante esse per?odo com a dieta controle (DC).As dietas foram mantidas durante o per?odo de acasalamento, gesta??o e lacta??o de todos os animais. Ap?s o nascimento da prole, foram retirados 10 filhotes de cada grupo para an?lise histopatol?gica dos germes dent?rios dos incisivos inferiores. Os demais passaram pela lacta??o at? completarem 60 dias de vida onde foram sacrificados e os dentes incisivos inferiores excisados para an?lise por microscopia eletr?nica de varredura (MEV) das altera??es estruturais, bem como para an?lise de c?lcio por absorb?ncia at?mica, do f?sforo pelo m?todo do vanadato-molibdato e de outros minerais pelo EDX. O f?gado dos animais foi removido para a an?lise das prote?nas hep?ticas. O estudo histopatol?gico mostrou que ao primeiro dia de vida, todos os animais apresentavam os incisivos inferiores, 90% estavam na fase de aposi??o e calcifica??o e em 60% destes foi poss?vel observar a forma??o de dentina. Na an?lise pelo MEV foi detectado em 90% dos animais do grupo DH que as extremidades dos incisivos inferiores estavam quebradi?as e sem contorno definido; neste mesmo grupo foram detectadas microfendas extensas, 369,66 nm? 3,45, enquanto que no grupo DC as fendas eram de 174 nm?5,72.. Em rela??o ?s concentra??es de c?lcio e f?sforo houve redu??o significativa no grupo DH e para os demais minerais, quase todos se apresentaram diminu?dos neste grupo exceto o Cl e o K que no esmalte apresentou valores superiores ao grupo DC. A diminui??o do aporte prot?ico influenciou significativamente no peso e no tamanho da prole, no entanto as prote?nas hep?ticas n?o demonstraram diferen?a significativa entre os grupos, levando-se a crer que foi induzida nestes animais uma desnutri??o prot?ica do tipo marasm?tica
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12632 |
| Date | 17 December 2003 |
| Creators | Lima, Hilk?a Carla de Souza Medeiros |
| Contributors | CPF:00443085404, http://lattes.cnpq.br/0129976807551158, Oliveira, Fernanda Wanderley de, CPF:45275831404, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786179U0&dataRevisao=null, Carvalho, Rejane Andrade de, CPF:15075079415, http://lattes.cnpq.br/7060394224697599, Lima, Dilma Ferreira |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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