The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T. aestivum X Ae. caudata and Ae. caudata, which was absent in all six addition lines. Each of these two primers produced a chromosome marker that could be tentatively located to the chromosome CA of Ae. caudata. OPJ-02, OPD-12, OPD-02, OPJ-12, OPD-20, and OPJ-14 produced a marker each for CB, CC, CD, CE, CF, and CG, respectively. OPJ-09 produced C-genome chromosome-specific RAPD markers. Also, OPC-05 and OPJ-19 produced RAPDs from both wheat and Ae. caudata genomes.
| Identifer | oai:union.ndltd.org:UTAHS/oai:digitalcommons.usu.edu:etd-5575 |
| Date | 01 May 1995 |
| Creators | Wei, Ling |
| Publisher | DigitalCommons@USU |
| Source Sets | Utah State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | All Graduate Theses and Dissertations |
| Rights | Copyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact Andrew Wesolek (andrew.wesolek@usu.edu). |
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