Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins

Description

Yes / The proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal76

Links & Downloads

1. Youssef MM, Al-Omair MA and Picksley SM (2014) Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins. Arabian Journal of Chemistry. 7(3): 327-334.
2. http://hdl.handle.net/10454/10558
3. https://doi.org/10.1016/j.arabjc.2011.02.001

Tags

RECN

SMC

tn10 excision

Recombination

Recombination-deficient mutants

Double-strand breaks

DNA-repair

Chromosome condensation

Exonuclease-i

k-12

Identification

Mutations

Strains

Product

Additional Fields

Identifer	oai:union.ndltd.org:BRADFORD/oai:bradscholars.brad.ac.uk:10454/10558
Date	06 February 2011
Creators	Youssef, M.M., Al-Omair, M.A., Picksley, Stephen M.
Source Sets	Bradford Scholars
Language	English
Detected Language	English
Type	Article, Published version
Rights	(c) 2014 Elsevier. This is an Open Access article distributed under the Creative Commons BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/3.0/), CC-BY-NC-ND