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Previous issue date: 2015-10-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A cana-de-a??car ? uma importante cultura para nosso pa?s, que contribui com quase metade de toda a produtividade mundial. Muitos desafios s?o enfrentados pelas plantas, que muitas vezes n?o atingem todo o seu potencial gen?tico devido a presen?a de estresses bi?ticos e abi?ticos. Como consequ?ncia, esses estresses podem gerar esp?cies reativas de oxig?nio, que podem danificar o material gen?tico. Outra consequ?ncia do estresse ? a flora??o precoce, que diminui tamb?m a produtividade da cana-de-a??car. Neste contexto, este trabalho apresenta como objetivos caracterizar os genes scMUTM1 e scMUTM2, duas DNA glicosilases pertencentes ? via de reparo por excis?o de base (BER), al?m de identificar transcritos potencialmente relacionados com estresse e reparo de DNA, em duas variedades com fen?tipo contrastante para flora??o. A caracteriza??o das glicosilases incluiu a constru??o de cassetes de express?o de prote?nas, para posterior purifica??o e uso em ensaios enzim?ticos; constru??o de ?rvore filogen?tica e an?lise de promotor deste gene. Com a reconstru??o filogen?tica de MUTM foi poss?vel observar que h? o agrupamento desta sequ?ncia em um ramo com monocotiled?neas e outro com dicotiled?neas. Isto sugere que a duplica??o desta sequ?ncia pode ter ocorrido depois da diverg?ncia desses dois grupos. Com a an?lise do promotor deste gene, ? sugerido que, provavelmente, o gene ScMUTM1 pode estar sofrendo subfuncionaliza??o, uma vez que a regi?o promotora do gene ScMUTM2 apresenta uma maior quantidade de sequ?ncias regulat?rias relacionadas ao estresse. Por meio das an?lises de express?o g?nica utilizando microarranjos, se observou a superexpress?o de genes relacionados ao estresse de forma marcante em uma das condi??es de an?lise relacionadas com flora??o precoce. / Sugarcane is an important culture for Brazil that holds almost half of all worldwide productivity. Plants face many challenges, because of biotic and abiotic stresses presents in the production field, which could prevent plants from reaching their genetic potential. As consequence, those stresses can generate Reactive Oxygen Species ? ROS ? that can cause damages on DNA. Another consequence of stress is the early-flowering process, which contributes for a reduction on yield. In this context, the aim of this work is to characterize ScMUTM1 and ScMUTM2, two DNA glycosylases belonging to base excision repair pathway; and identify genes potentially related to stress and DNA repair in two sugarcane cultivars with contrasting flowering phenotypes. The characterization of the DNA glycosylases included the construction of vector to over express the recombinant proteins ScMUTM1 and ScMUTM2; they will be used in a near future to purification of these proteins and use in enzymatic assays. It was also made a phylogenetic reconstruction of this gene in plants and analysis of its promoter. With the phylogenetic analysis, it is possible to observe the presence of these genes grouped inside a branch with monocots and another one with dicots. This suggests that the duplication of this gene probably occurred after the separation of these two groups. The analysis of the promotor of MUTM shows of the presence of stress-related regulatory motifs at ScMUTM2 promoter, when compared with ScMUTM1. This may suggests that ScMUTM1 might be suffering sub functionalization process. After the analysis of microarrays data, it is observed an up-regulation from some stress-related genes in one of the conditions analyzed, related to early flowering process.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21013 |
| Date | 16 October 2015 |
| Creators | Medeiros, Amanda Larissa Marques de |
| Contributors | 13451112825, http://lattes.cnpq.br/4808910380593455, Dalmolin, Rodrigo Juliani Siqueira, 94020035091, http://lattes.cnpq.br/4065178015615979, Praxedes, Sidney Carlos, 02106448422, http://lattes.cnpq.br/6609231229691480, Calsa J?nior, Tercilio, 17417313809, http://lattes.cnpq.br/2775650529232362, Grangeiro, Thalles Barbosa, 31160166315, http://lattes.cnpq.br/3114375474778667, Scortecci, Katia Castanho |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM BIOQU?MICA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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