The respiratory system is a target for environmental oxidants from airborne pollution and
other contaminants. Chronic exposure may result in inflammatory conditions such as
allergies and asthma. The ability of the body to counteract oxidation is dependent on the
oxidative status of the individual, and can also benefit from the use of medicinal plant
preparations such as Withania somnifera (WS), which is accredited with antioxidant and antiinflammatory
properties. Selenium is an important cofactor of antioxidant enzymes such as
glutathione peroxidase. A deficiency of Se will result in decreased antioxidant enzymes
levels and a poor response to oxidative damage. The aim of this study was to determine the
antioxidant content and activity of WS, as well as its in vitro effects with seleno-L-methionine
(Se-Met), the major component of dietary selenium, in a physiologically relevant cell model
system. Little is known about the combinational effects of non-enzymatic and enzymatic
antioxidants i.e., WS in combination with Se-Met, and these effects were also quantified.
The antioxidant content (total phenolic (TPC) and flavonoid content (TFC)) and activity (2,2-
diphenyl-2-picrylhydrazyl (DPPH), trolox equivalent antioxidant capacity (TEAC)) and oxygen
radical absorbance capacity (ORAC) of a water extract of WS, as traditionally prepared and
used, was determined. The intracellular and total protection of WS alone (0-33.33 mg/ml)
and in combination with Se-Met (0-33.33 pg/ml) (range: ½-100x RDA) was evaluated in a
standard cell line, the SC-1 fibroblast cell line and in physiologically relevant primary cultures
of chick embryo lung cells (CELC) using the 2’,7’-dichlorofluorescein diacetate (DCFH-DA) assay. Water extracts of WS, as traditionally consumed, were found to possess significant
antioxidant content and activity. For TPC and TFC, WS was found to contain 4.84 g CE/100
g DW, and 355 mg CE/100 g DW respectively. For DPPH, the IC50 of WS was 56.34 mg/ml,
and the antioxidant activity of WS was 274.8 μmol TE/g. For TEAC, the IC50 of WS was 60.3
mg/ml, and the antioxidant activity of WS was 2.2 mmol TE/g. Using ORAC, the antioxidant
activity of WS was found to be 15.05 μM TE/g.
WS and Se-Met exhibited both pro-oxidant and antioxidant effects in the DCFH-DA assay
and this may be a function of concentration, as well as combinations of WS and Se-Met
ratios. Data indicates that both WS and Se-Met, alone and in combination, elicit protection
via the intracellular (at all concentrations) and extracellular (at higher concentrations) compartment of the SC-1 cell. A synergistic interaction is observed extracellularly between
WS and Se-Met, with mostly additive effects occurring intracellularly.
For CELC, WS and Se-Met alone both demonstrated total protective effects at almost all
concentrations, and intracellular protective effects at higher concentrations. The combination
of WS and Se-Met provided total and intracellular protection to the CELC at the highest
concentration combination. Antagonism between WS and Se-Met was observed at low
concentrations in the total protection assay, with weak synergism and antagonism occurring
at higher concentrations. Synergism between WS and Se-Met was observed at low
concentrations intracellularly, with antagonistic interactions occurring at higher
concentrations.
In conclusion, WS and Se-Met, alone and in combination at non-cytotoxic concentrations,
displayed significant cellular protection against oxidative damage and this was a function of
cell line, concentration and ratios between WS and Se-Met. / Dissertation (MSc)--University of Pretoria, 2013. / gm2014 / Anatomy / unrestricted
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:up/oai:repository.up.ac.za:2263/33161 |
| Date | 28 January 2014 |
| Creators | Venter, Claire Lynne |
| Contributors | Bester, Megan J. |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Dissertation |
| Rights | © 2013 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria |
Page generated in 0.0022 seconds