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Previous issue date: 2015-04-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Head and neck cancer represents a group of diseases affecting the face and upper aerodigestive tract. Early diagnosis of these neoplasms and their etiologic factors is crucial for patient?s survival and treatment. In the last decades, cause-and-effect relationship between some viruses and malignancies has been widely accepted. Recently, the role of Human papillomavirus (HPV) and Epstein-Barr virus (EBV) has been shown in the pathogenesis of oropharyngeal carcinoma, undifferentiated nasopharyngeal carcinoma and lymphomas. Nevertheless, the role of such viral agents in oral squamous cell carcinoma (OSCC) is still controversial. This study aimed to investigate HPV, EBV, p53 and Ki-67 in OSCC and oral lymphoma, considering clinical and histological features. The sample was composed of archived material (medical records and paraffin blocks of biopsied specimens) from patients with OSCC, oral lymphoma, and oral traumatic fibrous hyperplasia. The biopsied specimens and medical records were allocated into five groups: (1) 16 samples from patients with OSCC grade I (well-differentiated); (2) 16 samples from patients with OSCC grade II (moderately-differentiated); (3) 19 samples from patients with OSCC grade III (poorly-differentiated); (4) 14 samples from patients with oral non-Hodgkin lymphoma; and (5) 19 samples from patients with oral traumatic fibrous hyperplasia. Biopsied specimens were analyzed for HPV, EBV, p53 and Ki-67 expression by immunohistochemistry, and clinical data concerning age and gender of the patients, size and duration of the lesion as well as habits and comorbidities were collected. Marker expression was evaluated according to clinical features and histological grade of the tumors. There was no association of HPV and EBV with the tumors analyzed. Ki-67 expression was significantly lower in the fibrous hyperplasia group with no other significant differences. p53 expression was significantly lower in the lymphoma and fibrous hyperplasia groups than the OSCC groups, with no other significant differences. OSCC grade II and III were associated with male gender and tobacco smoking, while grade III was also associated with alcohol consumption. There was no association of the tumors with either chimarr?o or comorbidities. Duration of the lesion was inversely correlated to lesion size, which in turn was inversely correlated to HPV. EBV and HPV were positively correlated to each other, as was Ki-67 to lesion size and p53, which was correlated to HPV. / Os tumores malignos da regi?o de cabe?a e pesco?o constituem um grupo de doen?as que afeta a face e o trato aerodigestivo superior. O diagn?stico precoce dessas neoplasias e de seus fatores etiol?gicos ? de extrema import?ncia para a sobrevida e para o tratamento do paciente. Nas ?ltimas d?cadas, a rela??o de causa e efeito entre certos v?rus e tumores malignos tem sido amplamente aceita. Recentemente, foi relatado o papel do Human papillomavirus (HPV) e do Epstein-Barr virus (EBV) na patogenia do carcinoma espinocelular de orofaringe, no carcinoma indiferenciado de nasofaringe e em linfomas, sendo ainda controverso o papel desses agentes virais no carcinoma de c?lulas escamosas oral (CCEO). O presente estudo teve por objetivo investigar, por meio de exame imunoistoqu?mico, os v?rus HPV e EBV, bem como os marcadores Ki-67 e p53 em carcinomas e linfomas orais considerando fatores cl?nicos e histol?gicos. Esp?cimes de bi?psia arquivados em blocos de parafina e prontu?rios de pacientes portadores de les?es orais de CCEO, linfoma e hiperplasia fibroepitelial foram distribu?dos em cinco grupos: (1) 16 amostras de CCEO grau I (bem diferenciado); (2) 16 amostras de CCEO grau II (moderadamente diferenciado); (3) 19 amostras de CCEO grau III (pobremente diferenciado); (4) 14 amostras de linfoma n?o-Hodgkin e (5) 19 amostras de hiperplasia fibroepitelial. Os esp?cimes de bi?psia foram submetidos a processamento imunoistoqu?mico para HPV, EBV, p53 e Ki-67. Dados cl?nicos referentes a idade e sexo dos pacientes, tamanho e dura??o da les?o, comorbidades e h?bitos foram coletados dos prontu?rios. A express?o dos marcadores foi analisada de acordo com os aspectos cl?nicos e grau histol?gico dos tumores. N?o houve associa??o entre HPV e EBV com os tumores analisados. A express?o do Ki-67 foi significativamente menor no grupo hiperplasia fibroepitelial, n?o havendo outras diferen?as significativas entre os demais grupos. Os linfomas e as hiperplasias fibroepiteliais exibiram express?o de p53 significativamente menor que os carcinomas, sem outras signific?ncias. CCEO grau II e III foram associados ao sexo masculino e ao tabagismo, enquanto o CCEO grau III foi tamb?m associado ao consumo de ?lcool. N?o houve associa??o dos tumores avaliados com chimarr?o ou comorbidades. Houve correla??o negativa entre dura??o e tamanho da les?o, bem como entre tamanho da les?o e HPV. EBV e HPV exibiram correla??o positiva, assim como o Ki-67 foi correlacionado ao tamanho da les?o e ? p53, que por sua vez exibiu correla??o positiva com o HPV.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6212 |
| Date | 28 April 2015 |
| Creators | Jornada, Vanessa Chidiac |
| Contributors | Cherubini, Karen |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, Brasil, Faculdade de Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | -8096554818733665164, 600, 600, 600, 600, 4673435736271820140, -2070498469879244349, 2075167498588264571 |
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