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Previous issue date: 2017-07-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O controle qu?mico do biofilme dent?rio e da microbiota planct?nica atua como
coadjuvante do controle mec?nico para fins de manuten??o e/ou restabelecimento da sa?de
bucal. Nesse contexto, emergem os fitoter?picos com a finalidade de atuar como agentes
antimicrobianos na cavidade oral. Diante disso, o presente estudo buscou avaliar a a??o
antimicrobiana dos extratos da folha e da raiz da Turnera ulmifolia Linn. (chanana) e da
folha da Passiflora edulis Flavicarpa Degener (maracuj?-amarelo) frente a bact?rias do
g?nero streptococcus (Streptococcus mutans, Streptococcus oralis e Streptococcus
sanguinis) e microrganismos superinfectantes (Pseudomonas aeruginosa e Enterococcus
faecalis) do ambiente bucal. Para isso foram investigadas a Concentra??o Inibit?ria
M?nima (CIM), a Concentra??o Inibit?ria M?nima de Ader?ncia (CIMA) e a Cin?tica
Bactericida dos extratos mencionados, utilizando como controle o digluconato de
clorexidina a 0,12%. No que diz respeito ? atividade inibidora de crescimento bacteriano
dos extratos investigados, verificou-se que frente ao Streptococcus mutans, o extrato da
folha da Turnera ulmifolia Linn se apresentou estatisticamente superior aos demais
(p<0,05); com rela??o aos Streptococcus oralis e Streptococcus sanguinis, foi o extrato da
folha da Passiflora edulis Flavicarpa Degener que exibiu resultados com signific?ncia
estat?stica (p<0,05) e diante dos microrganismos superinfectantes, foi o extrato da raiz da
Turnera ulmifolia Linn que apresentou os melhores resultados (p<0,05). Esses extratos
vegetais mostraram halos de inibi??o variados, apresentando at? 22mm de di?metro. No
tocante ao efeito antiaderente, todos os extratos testados apresentaram resultados positivos
em diferentes dilui??es, para todos os microrganismos estudados, merecendo destaque
apenas o efeito antiaderente do extrato da folha da Passiflora edulis Flavicarpa Degener
frente aos Streptococcus oralis e Streptococcus sanguinis. Quanto ? Cin?tica Bactericida, os
extratos bruto e dilu?dos testados n?o demonstraram atividade inibidora de crescimento bacteriano a
partir de 4h de contato com nenhum dos microrganismos do estudo. Por?m, o digluconato de
clorexidina a 0,12% se mostrou mais eficaz do que todos os referidos extratos para todos os testes
realizados, exceto para o efeito antiaderente do extrato da folha da Passiflora edulis Flavicarpa
Degener frente aos Streptococcus oralis e Streptococcus sanguinis, sendo esses ?ltimos superiores ao
digluconato de clorexidina a 0,12%. Portanto, conclui-se que os extratos vegetais apresentam
atividade inibidora de crescimento bacteriano, efeito antiaderente e potencial bactericida
inferiores ao digluconato de clorexidina a 0,12% frente a todos os microrganismos
estudados, exce??o feita somente ao efeito antiaderente do extrato da Passiflora edulis
Flavicarpa Degener com rela??o aos Streptococcus oralis e Streptococcus sanguinis que
parece ser superior ?quele do digluconato de clorexidina a 0,12% para os extratos
investigados. / The chemical control of the dental biofilm and the planktonic microbiota acts as a coadjuvant
of the mechanical control for maintenance and / or restoration of oral health. In this context,
phytotherapics emerge with the purpose of acting as antimicrobial agents in the oral cavity.
Therefore, the present study aimed to evaluate the antimicrobial action of leaf and root
extracts of Turnera ulmifolia Linn (Chanana) and the leaf of the Passiflora edulis Flavicarpa
Degener (yellow passion fruit) against bacteria of the genus Streptococcus mutans,
Streptococcus oralis and Streptococcus sanguinis and superinfecting microorganisms
(Pseudomonas aeruginosa and Enterococcus faecalis) from the oral environment. For this
purpose, the Minimal Inhibitory Concentration (MIC), Minimum Inhibitory Adhesion
Concentration (MIAC) and Bactericidal Kinetics of the mentioned extracts were investigated
using 0,12% chlorhexidine digluconate as the control. As regards the bacterial growth
inhibitory activity of the extracts investigated, it was verified that, in relation to Streptococcus
mutans, Turnera ulmifolia Linn leaf extract was statistically superior to the others (p<0,05), in
relation to Streptococcus oralis and Streptococcus sanguinis, was the extract of the leaf of the
Passiflora edulis Flavicarpa Degener that showed results with statistical significance
(p<0,05) and in front of the superinfecting microorganisms, was the root extract of Turnera
ulmifolia Linn that presented the best results (p<0,05). These plant extracts showed varied
inhibition halos, presenting up to 22mm in diameter. Regarding the anti-adherence effect, all
the extracts tested showed positive results at different dilutions for all the microorganisms
studied, with emphasis only on the non-stick effect of Passiflora edulis Flavicarpa Degener
leaf extract against Streptococcus oralis and Streptococcus sanguinis. Regarding Bactericidal
Kinetics, the crude and diluted extracts tested did not demonstrate bacterial growth inhibitory
activity after 4 hours of contact with any of the microorganisms in the study. However, the
chlorhexidine digluconate 0,12% was more effective than all the extracts for all tests, except
for the non-stick effect of Passiflora edulis Flavicarpa Degener leaf extract front of
Streptococcus oralis and Streptococcus sanguinis, and those higher than the 0,12%
chlorhexidine digluconate. Therefore, it is concluded that plant extracts presents inhibitory
activity of bacterial growth, non-stick effect and bactericidal potential lower than the 0,12%
compared chlorhexidine gluconate to all microorganisms studied, except only to the non-stick
effect of the extract of Passiflora edulis Flavicarpa Degener with respect to Streptococcus
oralis and Streptococcus sanguinis which appears to be superior to that of the 0,12%
chlorhexidine digluconate for the investigated extracts.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/24611 |
| Date | 25 July 2017 |
| Creators | Mendon?a, Ang?lica Kercya Pereira de |
| Contributors | 87808838420, Gurgel, Bruno C?sar de Vasconcelos, 02569549410, Gad? Neto, C?cero Rom?o, 50430947453, Lins, Ruthineia Di?genes Alves Uchoa |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM SA?DE COLETIVA, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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