Transforming growth factor beta 3 (TGF3) promotes tenogenic differentiation and
may enhance tendon regeneration in vivo. This study aimed to apply TGF3 absorbed in
decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity
of scaffold-associated TGF3 in an in vitro model. TGF3 could effectively be loaded onto tendon
scaffolds so that at least 88% of the applied TGF3 were not detected in the rinsing fluid of the
TGF3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells
(MSC) were then seeded on scaffolds loaded with 300 ng TGF3 to assess its bioactivity. Both
scaffold-associated TGF3 and TGF3 dissolved in the cell culture medium, the latter serving as
control group, promoted elongation of cell shapes and scaffold contraction (p < 0.05). Furthermore,
scaffold-associated and dissolved TGF3 affected MSC musculoskeletal gene expression in a similar
manner, with an upregulation of tenascin c and downregulation of other matrix molecules, most
markedly decorin (p < 0.05). These results demonstrate that the bioactivity of scaold-associated
TGF3 is preserved, thus TGF3 application via absorption in decellularized tendon scaffolds is a
feasible approach.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:89634 |
| Date | 09 February 2024 |
| Creators | Roth, Susanne Pauline, Brehm, Walter, Groß, Claudia, Scheibe, Patrick, Schubert, Susanna, Burk, Janina |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 5474 |
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