Cui, Ju. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 110-136). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.viii / Table of Contents --- p.ix / List of Tables and Figures --- p.xi / List of Abbreviations --- p.xiii / Chapter 1 General introduction --- p.1 / Chapter 2 Literature review --- p.4 / Chapter 2.1 --- Introduction --- p.4 / Chapter 2.2 --- Structural changes in ovary and thelycum of penaeid shrimp during maturaiton --- p.4 / Chapter 2.3 --- Biochemical changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 2.4 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.9 / Chapter 2.4.1 --- Peptides --- p.9 / Chapter 2.4.2 --- Steroids --- p.17 / Chapter 2.4.3 --- Terpenoids --- p.19 / Chapter 2.4.4 --- Biogenic amines --- p.21 / Chapter 2.6 --- Reproductive biology of the shrimp Metapenaeus ensis --- p.23 / Chapter 2.6 --- Proteomics --- p.27 / Chapter Chapter 3 --- Identification of proteins differentially expressed during ovarian maturation in the shrimp Metapenaeus ensis --- p.28 / Chapter 3.1 --- Introduction --- p.28 / Chapter 3.2 --- Materials and methods --- p.30 / Chapter 3.2.1 --- Animals --- p.30 / Chapter 3.2.2 --- Histological observation --- p.30 / Chapter 3.2.3 --- Data analysis --- p.31 / Chapter 3.2.4 --- Two-dimensional gel electrophoresis --- p.31 / Chapter 3.2.5 --- Protein isolation and identification by MALDI-TOF MS/MS --- p.35 / Chapter 3.3 --- Results --- p.37 / Chapter 3.3.1 --- General ovarian histology --- p.37 / Chapter 3.3.2 --- Morphometric analysis --- p.41 / Chapter 3.3.3 --- Comparison of proteomic patterns of shrimp ovaries and image analysis --- p.43 / Chapter 3.3.4 --- Identification of differentially expressed proteins in shrimp ovaries by MALDI-TOF MS/MS analysis --- p.48 / Chapter 3.4 --- Discussion --- p.61 / Chapter 3.4.1 --- Identification of proteins by MALDI-TOF MS/MS analysis --- p.61 / Chapter 3.4.2 --- Potential functions of the identified ditterentially expressed proteins in shrimp reproduction --- p.62 / Chapter Chapter 4 --- Characterization of cellular retinoic acid binding protein (CRABP) and retinoic X receptor (RXR) in the shrimp Metapenaeus ensis --- p.69 / Chapter 4.1 --- Introduction --- p.69 / Chapter 4.2 --- Materials and methods --- p.72 / Chapter 4.2.1 --- Experimental animals --- p.72 / Chapter 4.2.2 --- Preparation of total RNA --- p.72 / Chapter 4.2.3 --- Rapid Amplification of 5' and 3' cDNA Ends (RACE) --- p.73 / Chapter 4.2.4 --- Subcloning --- p.75 / Chapter 4.2.5 --- Sequencing --- p.78 / Chapter 4.2.6 --- Phylogenetic analysis --- p.78 / Chapter 4.2.7 --- Reverse transcription (RT) --- p.79 / Chapter 4.2.8 --- Semi-quantitative RT-PCR --- p.79 / Chapter 4.2.9 --- Real-Time RT-PCR --- p.80 / Chapter 4.2.10 --- In situ hybridization --- p.81 / Chapter 4.2.11 --- Tissue culture and in vitro ovary explant assay --- p.83 / Chapter 4.2.12 --- Statistical analysis --- p.84 / Chapter 4.3 --- Results --- p.84 / Chapter 4.3.1 --- Full-length RXR cDNA derivation --- p.84 / Chapter 4.3.2 --- Sequence comparisons and phylogenetic analyses --- p.86 / Chapter 4.3.3 --- Spatiotemporal expression profiles of MeCRABP and MeRXR mRNA in female shrimp --- p.92 / Chapter 4.3.4 --- Effect of exogenous retinoic acids on the expression of MeCRABP and MeRXR in shrimp ovaries --- p.96 / Chapter 4.4 --- Discussion --- p.101 / Chapter 4.4.1 --- Cloning and characterization of the M. ensis RXR --- p.101 / Chapter 4.4.2 --- Developmental expression of CRABP and RXR in shrimp ovary --- p.102 / Chapter 4.4.3 --- Effects of exogenous retinoic acids --- p.104 / Chapter Chapter 5 --- General conclusion --- p.106 / References --- p.110
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326081 |
| Date | January 2007 |
| Contributors | Cui, Ju., Chinese University of Hong Kong Graduate School. Division of Biology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiv, 136 leaves : ill. (some col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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