by Suen Yick-keung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1991. / Bibliography: leaves 163-170. / Abstract --- p.i / Acknowledgements --- p.v / Abbreviations --- p.vi / Objective of the study --- p.vii / Contents --- p.viii / Chapter Section 1 --- Introduction / Chapter 1. --- Roles of macrophges in immune system --- p.3 / Chapter 2. --- Special features of macrophages --- p.6 / Chapter 3. --- Transmembrane signalling in mammalian cells --- p.9 / Chapter 4. --- Transmembrane signalling in macrophages --- p.32 / Chapter 5. --- Choice of the macrophages for the study --- p.40 / Chapter Section 2 --- Materials and Methods / Materials / Chapter 1. --- Animals --- p.44 / Chapter 2. --- Chemicals --- p.44 / Chapter 3. --- Reagents --- p.45 / Methods / Chapter 1. --- pell culture --- p.47 / Chapter 2. --- 3H-thymidine incorporation --- p.48 / Chapter 3. --- Cytosolic free calcium determination --- p.48 / Chapter 4. --- Intracellular pH mesurement --- p.50 / Chapter 5. --- Determination of membrane potential --- p.50 / Chapter 6. --- Determination of phagocytic activity --- p.51 / Chapter 7. --- Cell adhesion assay --- p.52 / Chapter 8. --- Statistical analysis --- p.52 / Chapter Section 3 --- Results / Chapter 1. --- Effect of membrane potential on cell proliferation in PU5-1.8 cells and bone marrow-derived macrophages / Evidence for induction of cell proliferation mediated by membrane depolarization in PU5-1.8 cells --- p.53 / Evidence of an array of agonists on cell proliferation and membrane potential in PU5-1.8 cells --- p.57 / Interrelationship between membrane potential and FCS-mediated proliferation in PU5-1.8 cells --- p.61 / Cytosolic alkalinization induces membrane depolarization in PU5-1.8 cells --- p.64 / Suppression of membrane depolarization and cell proliferation by protein kinase C activation --- p.66 / Effect of membrane potentials on cell proliferation in bone marrow-derived macrophages --- p.68 / Chapter 2. --- Intracellular signals for the regulation of phagocytosis in PU5-1.8 cells / Phagocytosis of unopsonized yeast in PU5-1.8 cells --- p.71 / Effect of membrane potential on phagocytosis in PU5-1.8 cells --- p.73 / Changes in phagocytic activities in PU5-1.8 cells by activation of protein kinase C --- p.82 / Effects of protein kinase C on membrane potential- induced enhancement of phagocytosis in PU5-1.8 cells --- p.84 / Phagocytosis in PU5-1.8 cells requires assembly of microtubule --- p.90 / Effects of intracellular calcium and cAMP on phagocytosis in PU5-1.8 cells --- p.93 / Acidic intracellular pH enhances phagocytosis in PU5-1.8 cells --- p.98 / Chapter 3. --- Effects of various agonists on phagocytosis of yeast in PU5-1.8 cells / Effect of chemotactic peptide N-formyl- methionyl-leucyl-phenylalanine (FMLP) on phagocytosis in PU5-1.8 cells --- p.100 / Effects of lipopolysaccharide (LPS) on phagocytosis in PU5-1.8 cells --- p.105 / Effects of concanavalin A (Con A) on phagocytosis in PU5-1.8 cells --- p.109 / Effect of complement components on phagocytosis in PU5-1.8 cells --- p.113 / Chapter 4. --- Signal pathways for the regulation of cell adhesion on plastic surface in PU5-1.8 cells / Adhesion of PU5-1.8 cells on plastic surface --- p.119 / Effects of membrane potential on cell adhesion on plastic surface in PU5-1.8 cells --- p.121 / Effects of activation of protein kinase C on cell adhesion on plastic surface in PU5-1.8 cells --- p.125 / Effects of intracellular calcium and cAMP on adhesion on plastic surface in PU5-1.8 cells --- p.129 / In vivo cell adhesion of PU5-1.8 cells in Balb/c mice --- p.133 / Chapter 5. --- Effects of various agonists on the cell adhesion on plastic surface in PU5-1.8 cells / Dose dependent of various agonists against the cell adhesion ability of PU5-1.8 cells --- p.141 / Chapter 6. --- Cell adhesion to plastic surface in bone marrow-derived macrophages / Membrane potentials control the adhesiveness of bone marrow-derived macrophages (BMDM0) to plastic surface --- p.143 / Effects of phorbol ester PMA on cell adhesion to plastic surface in bone marrow-derived macrophages --- p.143 / "Effects of cAMP, [Ca2+ ]i and microtubule assembly on cell adhesion to plastic surfacein bone marrow-derived macrophages" --- p.146 / Chapter Section 4 --- Discussion / Chapter 1. --- Effects of membrane potential on cell proliferation in PU5-1.8 cells and bone marrow-derived macrophages --- p.148 / Chapter 2. --- Intracellular signals for the regulation of phagocytosis in PU5-1.8 cells --- p.151 / Chapter 3. --- Signal pathways for the regulation of cell adhesion on plastic surface in PU5-1.8 cells and bone marrow-derived macrophages --- p.158 / Chapter 4. --- General discussion --- p.161 / Chapter Section 5. --- Bibliography / References --- p.163
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318793 |
| Date | January 1991 |
| Contributors | Suen, Yick-keung., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, x, 170 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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