The microbial degradation of pyrene, a 4-ring polycyclic aromatic hydrocarbon (PAH), has been elucidated with the increasing number of pyrene-degrading bacteria that have been isolated in recent years. A pyrene degrading bacterium identified as Mycobacterium sp. strain S65, was isolated from a jet-fuel contaminated site in Sept-Iles, northern Quebec, Canada. S65 utilized pyrene, phenanthrene, and fluoranthene as sole carbon and energy sources, but did not degrade naphthalene, anthracene, and fluorene. Pyrene mineralization was enhanced by adding benz[a]anthracene, benzy[a]pyrene, or phenanthrene as cosubstrates. When added to PAH contaminated soil as a potential bioaugmentation agent, S65 did not appear to survive well, nor was it effective at degrading PAHs under these conditions. / Pyrene catabolic genes in S65 were partially characterized by Southern hybridization using a probe constructed from the naphthalene inducible pyrene dioxygenase gene, nidA, from the pyrene-degrading bacterium, Mycobacterium sp. strain PYR-1.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.79125 |
| Date | January 2002 |
| Creators | Sho, Michiei, 1976- |
| Contributors | Greer, Charles W. (advisor), Hamel, Chantal (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Natural Resource Sciences.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001985770, proquestno: AAIMQ88294, Theses scanned by UMI/ProQuest. |
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