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Previous issue date: 2009-04-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This study was conducted at the Laboratory of Pathology and Epidemiology, Department of
Plant Science of UFRRJ and at PESAGRO-RIO / Serop?dica Experimental Station with the
objective of selecting Trichoderma spp. antagonistic to Rhizoctonia in vitro; to quantify the
effect of Trichoderma spp. in protecting seeds and seedlings of tomato against the attack of
Rhizoctonia spp.; evaluate the effect of Trichoderma spp. in the emergence and quality of
tomato seedlings developed in the presence of the pathogen and determine the macronutrient
seedlings grown in a substrate inoculated with Trichoderma spp. and Rhizoctonia; study the
effect of the combination of thermal and chemical treatments with microbiolization seeds with
previously selected isolates of Trichoderma spp. and evaluate the survival of Trichoderma
spp. in spermosphere and in the rhizosphere of seedlings. Were used isolates of Trichoderma
spp, pre-selected from seeds of Santa Clara Miss Brazil , Garrafinha Vermelho and
Perinha ?gua Branca , untreated with fungicide. Three of the isolates showed antagonistic
activity in vitro and suppression of colony growth of Rhizoctonia spp. In tests of biological
control under greenhouse conditions, effects of treatments were seen in the emergence, length
and dry weight of shoot and root, root area of plants, teor and content of nitrogen, phosphorus
and potassium, and easy recovery of Trichoderma spp. from seeds and roots of seedlings. For
combination of thermal and chemical treatments of the seeds with Trichoderma spp. there
went significant effects of treatments on emergence and seedling development. The seed
treatment via dry heat at 70 ? C / 96 hours resulted in low germination both in Gerbox? and
in the substrate, while treatments with water heated to 50 ? C for 30 minutes and with HCl 5%
per 10 minutes were the least damage caused to the seeds. Seed treatment with hot water
associated with isolated or TENA7 TENA16 as biological protectors resulted in good
emergence and development of seedlings and saplings, as well as the association between
treatment with 5% HCl for 10 minutes followed by application of Trichoderma isolate
TENA7. / O presente trabalho foi realizado no Laborat?rio e Epidemiologia e Patologia de Sementes do
Departamento de Fitotecnia da UFRRJ e na PESAGRO-RIO/ Esta??o Experimental de
Serop?dica com o objetivo de selecionar isolados de Trichoderma spp. antagonistas a
Rhizoctonia in vitro; quantificar o efeito da aplica??o de Trichoderma spp. na prote??o de
sementes e pl?ntulas de tomate contra o ataque de Rhizoctonia spp; .avaliar o efeito da
aplica??o de Trichoderma spp. na emerg?ncia e qualidade das mudas de tomate desenvolvidas
na presen?a do pat?geno; determinar os teores de macronutrientes das mudas formadas em
substrato inoculados com isolados de Trichoderma spp. e Rhizoctonia; estudar o efeito da
associa??o de tratamentos t?rmicos e qu?mico com a microbioliza??o das sementes com
isolados previamente selecionados de Trichoderma spp. e avaliar a sobreviv?ncia de isolados
de Trichoderma spp. na espermosfera e rizosfera das mudas. Utilizaram-se isolados de
Trichoderma spp. previamente selecionados e sementes das cultivares Santa Clara Miss Brasil
e Garrafinha Vermelho sem tratamento pr?vio com fungicida. Tr?s dos isolados testados
apresentaram antagonismo in vitro e supress?o do crescimento das col?nias de Rhizoctonia
spp. Nos ensaios de controle biol?gico sob condi??es de casa-de-vegeta??o, foram observados
efeitos dos tratamemtos na emerg?ncia, comprimento e massa seca de parte a?rea e ra?zes,
?rea radicular das mudas, nos teores e conte?dos de N, P e K e f?cil recupera??o dos isolados
de Trichoderma spp. a partir das sementes e ra?zes das mudas. Na associa??o de tratamentos
t?rmico e qu?mico das sementes com isolados de Trichoderma spp. houve efeito significativo
dos tratamentos sobre a emerg?ncia e desenvolvimento das mudas. O tratamento das
sementes via calor seco a 70?C/ 96 hs resultou em baixa germina??o de sementes tanto, em
gerbox quanto em substrato, enquanto os tratamentos com ?gua aquecida a 50? C por 30 min e
com HCl a 5% por 10 min foram os que menos danos causaram ?s sementes. O tratamento de
sementes com ?gua quente associado aos isolados TENA7 ou TENA16 como protetores
biol?gicos resultaram em boa emerg?ncia e desenvolvimento das pl?ntulas e mudas, assim
como a associa??o entre tratamento com HCl a 5% por 10 min seguido da aplica??o do
isolado TENA7 de Trichoderma.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/556 |
| Date | 28 April 2009 |
| Creators | Sudo-martelleto, Mariluci |
| Contributors | Carmo, Margarida Gor?te Ferreira do, Carvalho, Aldir de Oliveira de |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Fitotecnia, UFRRJ, Brasil, Produ??o Vegetal |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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