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A salivary chromogenic assay for periodontitis

Periodontitis tends to be associated with sulfur-producing bacteria. This study uses a chromogenic sulfur detecting strip to compares whole saliva sulfur (SS) scores in subjects with and without periodontal diseases and examines the relationship between SS scores and clinical measures of periodontal status.Materials & Methods: Ninety-sex subjects were enrolled, mean age (SD) 36.5 (15.42), age range 22 to 82, percent males 52%, percent Caucasians 57%, percent cigarette smokers 33%. Periodontal evaluations including plaque index (PI), gingival index (GI), probing depth measurements (PD) and attachment levels (AL) were performed on 6 sites per tooth. Subjects were divided into 3 groups: periodontally healthy (no loss of attachment and no gingival bleeding, 17 subjects), gingivitis (no loss of attachment and presence of gingival bleeding, 54 subjects) and periodontitis (loss of attachment > 5 mm in 5 or more teeth, 25 subjects). A single chromogenic strip was used to collect a whole saliva sample from the mouth. Color reaction was scored based on a color chart. The scoring examiner was blinded to clinical status. Results: Good to moderate correlations were found between SS scores and PI (r=0.47, p=0.0001), GI (r=0.45, p=0.0001), PD (r=0.42, p=0.0001), and AL (r=0.30, p=0.002). Analysis of variance showed significant differences in SS scores among the three study groups (p = 0.0001); post-hoc analysis showed higher SS scores in periodontitis subjects than non-periodontitis subjects (p = 0.05). Nominal logistic regression adjusting for smoking showed the odds ratio of periodontitis increase by a factor of 12.76 for each increase of one unit of measure of SS. Conclusion: The results suggest that assessing whole saliva sulfur levels with a chromogenic strip has potential as a screening test for periodontal diseases. / Oral Biology

Identiferoai:union.ndltd.org:TEMPLE/oai:scholarshare.temple.edu:20.500.12613/2347
Date January 2011
CreatorsSeyedain, Merriam
ContributorsKhocht, Ahmed, Suzuki, Jon, 1947-, Yang, Jie
PublisherTemple University. Libraries
Source SetsTemple University
LanguageEnglish
Detected LanguageEnglish
TypeThesis/Dissertation, Text
Format31 pages
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Relationhttp://dx.doi.org/10.34944/dspace/2329, Theses and Dissertations

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