The global demand for nitrogen fertilizer was 12 million metric tonnes in 2014 and is expected to increase to 240 million metric tonnes by the year 2050, with the growth of the global population. To meet the demand for nitrogen fertilizers, the Haber-Bosch process has primarily been used to produce the precursor of many nitrogen fertilizers - ammonia. The very energy-expensive Haber-Bosch process uses fossil fuels and, therefore, a renewable source of ammonia must be established. Some microorganisms can use atmospheric nitrogen to produce ammonia via the nitrogenase enzyme, a mechanism attractive for alternative ammonia production. In this thesis project, integrating vectors for the marine cyanobacterium Synechococcus PCC 7002 have been designed and generated to facilitate future heterotrophic nitrogenase integration and ammonia production. The vectors were designed for integration in seven neutral sites of the Synechococcus PCC 7002 genome. Five of the seven planned integrating vectors were successfully constructed and transformation was attempted into Synechococcus PCC 7002 to determine the transformation efficiency of the different neutral sites, however, the transformation results were inconclusive.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-530245 |
| Date | January 2024 |
| Creators | Jennersjö Hedman, Alma |
| Publisher | Uppsala universitet, Institutionen för biologisk grundutbildning |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf, application/pdf |
| Rights | info:eu-repo/semantics/openAccess, info:eu-repo/semantics/openAccess |
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