Ex vivo expansion of T lymphocytes is a central process in the generation of cellular therapies
targeted at tumors and other disease-relevant structures,which currently cannot be reached by
established pharmaceuticals. The influence of culture conditions on T cell functions is, however,
incompletely understood. In clinical applications of ex vivo expanded T cells, so far, a relatively
classical standard cell culture methodology has been established. The expanded cells have
been characterized in both preclinical models and clinical studies mainly using a therapeutic
endpoint, for example antitumor response and cytotoxic function against cellular targets,
whereas the influence of manipulations of T cells ex vivo including transduction and culture
expansion has been studied to a much lesser detail, or in many contexts remains unknown.
This includes the circulation behavior of expanded T cells after intravenous application, their
intracellular metabolism and signal transduction, and their cytoskeletal (re)organization or their
adhesion, migration, and subsequent intra-tissue differentiation. This review aims to provide an
overview of established T cell expansion methodologies and address unanswered questions
relating in vivo interaction of ex vivo expanded T cells for cellular therapy.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:87516 |
| Date | 20 October 2023 |
| Creators | Sudarsanam, Harish, Buhmann, Raymund, Henschler, Reinhard |
| Publisher | Frontiers Media S.A. |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 886637 |
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