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Previous issue date: 2012-03-14 / Toxoplasmosis, a benign disease in normal healthy individuals, can have serious effects in pregnant women and immunocompromised patients. It is a parasitic disease caused by Toxoplasma gondii (Tg), an obligatory intracellular protozoan. The prophylactic and therapeutic arsenal against this parasite is very restricted. Thus, there is an ongoing search for novel drugs and therapeutic strategies. A promising alternative is a rational approach using medicinal plants. This study aimed to standardize methodologies for assessing the toxicological, antiproliferative, antioxidant, antiinflammatory and anti-Toxoplasma effects of Estragole and Thymol compounds isolated from species of plants (Lippia sidoides and Croton zenhtneri) commonly used in the Cariri region of Ceara State, Brazil. First we evaluated in vivo toxicity and conducted a pathological analysis of mice livers. In vivo antiinflammatory activity was assessed using air pouch and paw edema methods. Cytotoxicity assays were performed and antiproliferative, antioxidant and nitric oxide production analyzed. Anti-Toxoplasma activity was evaluated in a congenital experimental model with varying stages of maternal infection using the ME-49 strain and a non- congenital model by using ME-49 and RH strains. The results suggest low to moderate toxicity for both compounds. Thymol was more toxic in vivo and in vitro, having greater pathological repercussion than Estragole. The compounds were inactive for antiproliferative activity. Thymol showed better antioxidant activity, while Estragole stimulated nitric oxide production in macrophages. Both showed significant antiinflammatory activity. In non-congenital Tg infection, both compounds were active only against the ME49 strain. In congenital infection, Estragole (oral route) improved the newborn weight of infected mothers compared with untreated controls. Subcutaneous administration of the two compounds increased the weight of offspring born to infected mothers compared with untreated controls. We concluded that Estragole and Thymol exhibit important biological and anti-Toxoplasma activities. Further studies are needed to elucidate the mechanism of action of these compounds and other possible activities not investigated in the present study / A toxoplasmose, doen?a de curso benigno em pacientes imunocompetentes, mas que pode ocasionar graves acometimentos cl?nicos em imunossuprimidos e gestantes ? causada pelo Toxoplasma gondii (Tg), protozo?rio intracelular obrigat?rio. O arsenal profil?tico e terap?utico contra este parasito ? bastante restrito, tornando urgente a busca por novos medicamentos. Uma alternativa promissora ? a abordagem racional a partir de plantas medicinais. Neste estudo foram padronizadas metodologias objetivando avaliar os efeitos toxicol?gicos, antiproliferativos, antiinflamat?rios, antioxidantes e anti-toxopl?smicos do Timol e Estragol isolados, respectivamente, das esp?cies vegetais Lippia sidoides e Croton zenhtneri, usadas popularmente na regi?o do Cariri/CE para diversas mol?stias. Inicialmente foram realizados testes de toxicidade in vivo e an?lise patol?gica do f?gado dos camundongos. Avaliou-se a atividade antiinflamat?ria in vivo pelo m?todo da bolsa de ar e pela medida da varia??o do edema de pata. Ensaios de citotoxicidade in vitro foram realizados, bem como pesquisas de atividade antiproliferativa, antioxidante e produ??o de ?xido n?trico. A atividade anti-toxopl?smica foi avaliada em modelo murino de infec??o n?o cong?nita (usando Tg ME-49 e Tg RH) e cong?nita, com per?odos variados de infec??o materna (Tg ME-49). Os resultados sugerem toxicidade baixa a moderada para os dois compostos. O Timol se mostrou mais t?xico, in vivo e in vitro, e teve maior repercuss?o patol?gica do que o Estragol. Os compostos foram inativos para atividade antiproliferativa. O Timol apresentou ?tima atividade antioxidante enquanto o Estragol estimulou a produ??o de ?xido n?trico em macr?fagos. Ambos apresentaram significativa atividade antiinflamat?ria. Os dois compostos foram ativos apenas contra a cepa ME-49. No modelo de infec??o cong?nita o Estragol proporcionou um aumento significativo de peso neonatal em filhotes de m?es infectadas quando comparadas com o controle n?o tratado. O mesmo ocorreu com filhotes de m?es tratadas, via subcut?nea, com ambos os compostos ap?s 30 dias. Conclui-se que o Timol e o Estragol possuem importantes atividades biol?gicas e anti-toxopl?smica. Estudos posteriores s?o necess?rios para elucidar o mecanismo de a??o destes compostos, al?m de outras poss?veis atividades n?o investigadas neste estudo
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13081 |
| Date | 14 March 2012 |
| Creators | Oliveira, Claudio Bruno Silva de |
| Contributors | CPF:59626305487, http://lattes.cnpq.br/4863082845974813, Romero, Oscar Bruna, CPF:01525892606, http://lattes.cnpq.br/2472508053117517, Rachetti, Vanessa de Paula Soares, CPF:28762725807, http://lattes.cnpq.br/1524215726056886, Andrade Neto, Valter Ferreira de |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Biol?gicas, UFRN, BR, Biodiversidade; Biologia Estrutural e Funcional. |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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