Ion channels are proteins that facilitate ion diffusion across cell membrane.
Nevertheless, various groups of ion channels can act as surface receptors and play
important roles in signal transduction. Transient Receptor Potential Melastatin 7
(TRPM7) ion channel has been implicated in diverse cellular functions including
actomyosin cytoskeletal remodeling and anoxic neuronal death. However the
mechanisms behind TRPM7’s physiological roles remain undetermined. TRPM7
possesses unusually long intracellular domains and a functional C-terminal alpha kinase
domain that may contribute to regulation of channel activity and signal transduction. We
therefore identified proteins that interact with TRPM7 C-terminus. Pull-down assays
coupled with LC-MS/MS revealed that cytoskeletal proteins (actin and tubulin) and
synaptic vesicle proteins (VAMP2 and SNAP25) associate with the TRPM7. In addition,
we further found that TRPM7 does not directly bind microtubules or single dimeric
tubulin subunits. Thus one or more microtubule binding proteins is involved in the association between TRPM7 and microtubules.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/18245 |
| Date | 13 January 2010 |
| Creators | Chan, Chan |
| Contributors | Aarts, Michelle |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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