The purpose of this study was to compare the antimicrobial effect of MTAD, two of its components, doxycycline and citric acid, and NaOCl on E. faecalis in two in vitro models: the bovine tooth model and the agar diffusion model. In the tooth model, the lumens of thirty bovine dentin discs were standardized, autoclaved and infected with E. faecalis for 14 days. The infected dentin discs were treated with either MTAD, doxycycline 100mg/ml, 10% citric acid, 5.25% NaOCl or saline for 10 minutes. Dentinal shavings were collected using sterile 037 and 040 round burs. The number of bacteria in the shavings was enumerated by overnight CO2 culturing on TSA agar plates. For the agar diffusion model, E. faecalis was plated before placing paper discs wetted with three dilutions of each irrigant. The zone of inhibition was recorded after overnight culture. The data were analyzed with a two-way ANOVA and Tukey-Kramer post-hoc test. In the bovine tooth model, NaOCl and doxycycline were more effective than control in killing E. faecalis at the shallow bur depth but at the deeper bur depth, only NaOCl was significantly more effective (psE. faecalis.
| Identifer | oai:union.ndltd.org:vcu.edu/oai:scholarscompass.vcu.edu:etd_retro-1150 |
| Date | 01 January 2006 |
| Creators | Krause, Trisha Ann |
| Publisher | VCU Scholars Compass |
| Source Sets | Virginia Commonwealth University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Retrospective ETD Collection |
| Rights | © The Author |
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