<p> </p><p><em>Trypanosoma theileri</em>, of the subgenus <em>Megatrypanum, </em>a non-pathogenic cosmopolitan blood dwelling parasite of bovine. <em>T. theileri </em>can be cultured at room temperature in several culture media.</p><p>Blood samples were collected from deer's. To see if the blood was infected with trypanosomes it was cultivated in 2 ml sheep blood or cell cultivation medium DMEM with antibiotics.</p><p>Growth was detected by microscopy to see if there were any trypanosomes.</p><p>To determine the species of trypanosomes that was in the deer blood a DNA-preparation was done before a Polymerase Chain Reaction (PCR) could be done. With sequencing the trypanosomes where determined to be<em> Trypanosoma theileri</em>.</p><p>Different tests were made to see in what way the trypanosomes best were caught to the objective slides.</p><p>Forty samples of borrelia positive serum from forty different patients were tested with the fluorescent microscopy. Forty different samples from blood donors were tested the same way.</p><p>Blood samples from 16 different fissiped were taken and to see if they were infected with trypanosomes. Three different PCR's were done on the 16 blood samples.</p><p>A small test on human blood was also performed.</p><p>Protein identification by immunoblot with western blot and silver staining was done.</p><p>With the electron microscopy tests were done in the ordinary way and Critical Dry Point to see if both of the techniques worked.</p><p>Enzyme-Linked Immuno Sorbent Assay (ELISA) test were accomplished on two 96 well plates. The wells on the plates were diluted in different ways before they were processed.</p><p> </p><p> </p>
| Identifer | oai:union.ndltd.org:UPSALLA/oai:DiVA.org:mdh-6435 |
| Date | January 2009 |
| Creators | Bech, Linda |
| Publisher | Mälardalen University, School of Sustainable Development of Society and Technology |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, text |
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