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Previous issue date: 2015-11-12 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Este estudo conjuga uma abordagem etnofarmacol?gica no ?mbito do uso tradicional das esp?cies como triagem, em associa??o a aspectos filogen?ticos, considerando que h? comprova??es cient?ficas quanto ? composi??o qu?mica e atividades farmacol?gicas em outras esp?cies dentro dos mesmos g?neros. Isto est? associado a avalia??es das atividades antioxidante e hepatoprotetora j? realizado em estudos pr?vios do grupo de pesquisa usando o extrato bruto do Spondias mombin?S. tuberosa e Turnera ulmifolia Linn. var. elegans, que indicaram um efeito terap?utico positivo, al?m da da import?ncia de uma avalia??o toxicol?gica, outras atividades farmacol?gicas e a elucida??o dos compostos majorit?rios presentes nos extratos brutos e fra??es de Licania tomentosa Benth. Fritsch, C. impressa Prance, L. rigida Benth., S. mombim?S. tuberosa e T. ulmifolia Linn. var. elegans. Assim, os extratos das folhas e suas fra??es referentes ?s esp?cies supracitadas foram caracterizados quanto a composi??o qu?mica, atividades farmacol?gicas atrav?s de ensaios in vitro e ex vivo, al?m da avalia??o in vivo de sua toxicidade. A composi??o qu?mica dos extratos brutos e suas fra??es foram avaliadas por um m?todo cromatogr?fico validado e por t?cnicas espectrofotom?tricas, que possibilitam a identifica??o e/ou caracteriza??o de compostos majorit?rios. Esta an?lise indicou que os extratos e suas fra??es s?o uma fonte de compostos fen?licos, principalmente flavon?is e seus glicos?deos, tais como flavon?is-3-O-glicosilados. Em rela??o ? atividade antioxidante, apenas o extrato metan?lico das folhas de S. mombim?S. tuberosa e a FRF apresentaram uma acentuada capacidade de sequestro do radical livre DPPH?, enquanto que FRP foi ?nico capaz de proteger a integridade celular dos eritr?citos ao prevenir a hem?lise, inibindo a lipoperoxida??o l?pica e mantendo os n?veis de GSH. Os outros extratos induziram hem?lise. Este efeito antioxidante est? associado ? composi??o qu?mica presente em todos os extratos e fra??es. A avalia??o da atividade antimicrobiana s? apresentou um moderado efeito bactericida com o extrato de S. mombim?S. tuberosa e a FRF. A avalia??o toxicologica de todos os extratos em modelo murino, utilizando-se a dose ?nica do extrato na concentra??o de 2000 mg/kg, n?o mostrou qualquer efeito t?xico. Portanto, os resultados aqui descritos promovem um conhecimento cient?fico complementar, viabilizando o uso das folhas dessas esp?cies vegetais como recurso terap?utico, permitir o aproveitamento racional das mesmas, e um controle do extrativismo indiscriminado devido a usos medicinais n?o validados cientificamente, c? no Nordeste do Brasil e no mundo. / This study combines one ethnopharmacological approach within the traditional use of species such as screening, in association with phylogenetic aspects, considering the scientific evidences regarding the chemical composition and pharmacological activities in other species within the same genus. This is associated with assessments of antioxidant and hepatoprotective activities already performed in previous studies from the research group using the crude extract of Spondias mombim?S. tuberosa and Turnera ulmifolia Linn. var. elegans, which indicated a positive therapeutic effect, as well as the importance of a toxicological evaluation, other pharmacological activities and the elucidation of the major compounds present in crude extracts and fractions of Licania tomentosa Benth. Fritsch, C. impressa Prance, L. rigida Benth., S. mombim?S. tuberosa and Turnera ulmifolia Linn. var. elegans. Thus, leaf extracts and their fractions relating to the above mentioned species were characterized for chemical composition, pharmacological activities by in vitro and ex vivo assays, as well as the in vivo evaluation of toxicity. The chemical composition of crude extracts and fractions were evaluated by a validated chromatographic procedure and spectrometric techniques, which enabled the identification and/or characterization of compound majority. This analysis pointed that extracts and their fractions are a source of phenolic compounds, especially flavonoids and their glycosides such as flavonls-3-O-glicosilates. In the leaf extract of S. mombim?S. tuberosa and its rich in flavonoids fraction, called FRF, was isolated and characterized rutin as major compound of this species. Regarding the antioxidant activity, only the methanol S. mombim?S. tuberosa leaf extract and its FRF showed a marked DPPH free radical scavenging capacity, while FRP was only one able to protect the erythrocyte integrity by preventing its hemolysis by inhibiting lipid lipoperoxidation and maintaining the GSH levels. The other extracts induced hemolysis. This antioxidant effect is associated to chemical composition in all extracts and fractions. The antimicrobial activity assessment showed just a moderate bactericidal effect in the presence of the S. mombim?S. tuberosa extract and its FRP. The toxicological assessment of all extracts in a murine model, using a single dose of the extract at a concentration of 2000 mg / kg, showed no toxic effect. Therefore, the results described herein promote a complementary scientific knowledge, enabling the use of these plant species leaves as a therapeutic resource, allowing the rational use of the same, and control the indiscriminate extraction due to medical uses no scientifically validated, here in Northeastern Brazil and worldwide.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/21552 |
| Date | 12 November 2015 |
| Creators | Silva, Gabriel Ara?jo da |
| Contributors | 20054548420, Maciel, Maria Aparecida Medeiros, 37320165449, Langassner, Silvana Maria Zucolotto, 81995725072, Ara?jo, Thiago Ant?nio de Sousa, 00761490485, Moura, Tulio Fl?vio Accioly de Lima e, 13138863434, Almeida, Maria das Gra?as |
| Publisher | PROGRAMA DE P?S-GRADUA??O EM DESENVOLVIMENTO E INOVA??O TECNOL?GICA EM MEDICAMENTOS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Unknown |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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