The fungi are being opportunistic, with wide distribution in nature, including the
hospital environment, where it is frequent the presence of microorganisms that
cause hospital infection, especially in immunocompromised patients. To identify
and quantify the fungal microbiota in air, the filters of air-conditioners and
samples of newborns from the neonatal ICU at University Hospital / HUPA,
Maceió, Alagoas. Were held 20 exhibitions of collections totaling 400
disposable Petri dishes containing Sabouraud agar medium plus antibiotic. The
plates were placed randomly inside and open the two neonatal ICUs (A and B)
for 20 minutes. Also, there were 20 samples of the filters of air-conditioners of
neonatal ICUs and conducted the direct examination and culture of clinical
samples from newborns. CFU were isolated in 1209, these 675 (55.8%) were
isolated CFU before cleaning to be done in the neonatal ICU and 534 (44.2%)
CFU obtained after cleaning. Of 697 CFU of the environment of the NICU, 372
were isolated before and 325 after cleaning. Environment B, were obtained of
512 CFU and 303 CFU were obtained before cleaning and 209 after UFC.
During the study species of Cladosporium was the most representative in the air
and in the filters of air-conditioners. The clinical sample was more
representative urine with 79.5%. Among the identified yeast Candida
parapsilosis was present in 38.9% of environmental isolates of yeast and
Candida albicans was isolated in 20% of clinical samples of newborns. The
presence of pathogenic fungi in the two environments of the neonatal ICU and
clinical samples of infants, demonstrating the need for constant monitoring to
take control of microorganisms in hospital environments, especially in intensive
care units where there is the presence of immunocompromised patients prone
to develop fungal infections. / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Os fungos que são dispersos pelo ar atmosférico são denominados de fungos
anemófilos e são importantes por causar patologias em pacientes
imunocomprometidos. Com o objetivo de monitorar a microbiota fúngica do ar,
dos filtros dos condicionadores de ar e das amostras clínicas dos recémnascidos
internados na UTI neonatal do Hospital Universitário de Maceió - AL
foram realizadas coletas de amostras do ar através da técnica de exposição de
placas de Petri contendo o meio ágar Sabouraud acrescido de antibiótico. O
material sólido dos filtros dos condicionadores de ar foi coletado utilizando um
swab esterilizado. E das amostras clínicas dos recém-nascidos foram
realizados o exame direto e cultura. Para identificação dos fungos filamentos
foram comparadas as características macro e microscópicas e as leveduras
foram identificados através da técnica de PCR. Entre as 400 exposições de
placas realizadas foram isoladas 1029 UFC, sendo 675 (55,8%) UFC obtidas
antes e 534 (44,2%) após a limpeza. Das 40 amostras analisadas dos filtros
dos condicionadores de ar foram obtidas 303 UFC, sendo o fungo
Cladosporium cladosporioides com maior frequência tanto no ar com 186
(15,4%) UFC como nos filtros dos condicionadores de ar com 77 (37,7%). Não
foram isolados fungos filamentosos das amostras clínicas dos recém-nascidos.
Entretanto as leveduras Candida albicans, C. tropicalis e C. parapsilosis foram
obtidas tanto das amostras do ar como dos recém-nascidos com exceção de C.
guilliermondii que foi isolada apenas das amostras do ar.
| Identifer | oai:union.ndltd.org:IBICT/oai:www.repositorio.ufal.br:riufal/920 |
| Date | 17 July 2009 |
| Creators | Souza, Aryanna Kelly Pinheiro |
| Contributors | Silva Filho, Eurípedes Alves da, SILVA FILHO, E. A., Araújo, Maria Anilda dos Santos, ARAÚJO, M. A. S., Magalhães, Oliane Maria Correia, MAGALHÃES, O. M. C., Herrmann, Délia Maria de Moura Lima, HERRMANN, D. M. M. L., Silva, Luiz Antonio Ferreira da, SILVA, L. A. F. |
| Publisher | Universidade Federal de Alagoas, BR, Biologia, Programa de Pós-Graduação em Ciências da Saúde, UFAL |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFAL, instname:Universidade Federal de Alagoas, instacron:UFAL |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | bitstream:http://www.repositorio.ufal.br:8080/bitstream/riufal/920/1/Dissertacao_AryannaKellyPinheiroSouza_2009.pdf, bitstream:http://www.repositorio.ufal.br:8080/bitstream/riufal/920/2/Dissertacao_AryannaKellyPinheiroSouza_2009.pdf.txt |
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