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Spatial and seasonal variabilities of picoeukaryote communities in a subtropical eutrophic coastal ecosystem based on analysis of 18S rDNA sequences.

Cheung, Man Kit. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 81-93). / Abstracts in English and Chinese. / Abstract (English) --- p.I / Abstract (Chinese) --- p.III / Acknowledgements --- p.V / Table of contents --- p.VI / List of figures --- p.IX / List of tables --- p.XI / List of Appendices --- p.XII / Chapter Chapter 1. --- General introduction --- p.1 / Chapter 1.1. --- Picoeukaryotes --- p.1 / Chapter 1.2. --- Conventional characterization techniques --- p.1 / Chapter 1.3. --- Cloning and sequencing approach --- p.3 / Chapter 1.3.1. --- Applications in prokaryotic plankton --- p.3 / Chapter 1.3.2. --- Applications in eukaryotic picoplankton --- p.3 / Chapter 1.4. --- Variations in diversity with environmental factors --- p.5 / Chapter 1.5. --- Study site --- p.6 / Chapter 1.6. --- Objectives --- p.8 / Chapter Chapter 2. --- Materials and methods --- p.9 / Chapter 2.1. --- Study site --- p.9 / Chapter 2.2. --- Sample collection --- p.9 / Chapter 2.3. --- DNA extraction and 18S rRNA gene amplification --- p.11 / Chapter 2.4. --- Clone library construction and screening --- p.12 / Chapter 2.5. --- Sequencing and phylogenetic analysis --- p.13 / Chapter 2.6. --- Statistical analyses --- p.14 / Chapter Chapter 3. --- Results --- p.15 / Chapter 3.1. --- Hydrological parameters of study site --- p.15 / Chapter 3.2. --- Clone libraries --- p.15 / Chapter 3.3. --- Higher-level taxonomic distribution --- p.21 / Chapter 3.4. --- Phylogenetic affiliations of OTUs --- p.22 / Chapter 3.4.1 --- Alveolates --- p.35 / Chapter 3.4.2 --- Stramenopiles --- p.36 / Chapter 3.4.3 --- Rhizaria --- p.36 / Chapter 3.4.4 --- Other lineages --- p.37 / Chapter 3.4.5 --- Novel higher-level groups --- p.38 / Chapter 3.5. --- Diversity estimates of picoeukaryotes --- p.39 / Chapter Chapter 4. --- Discussion --- p.42 / Chapter 4.1. --- Picoeukaryotic diversity --- p.42 / Chapter 4.1.1 --- Overall diversity --- p.42 / Chapter 4.1.2 --- Diversity of individual taxonomic groups --- p.44 / Chapter 4.1.2.1 --- Most represented lineages --- p.44 / Chapter 4.1.2.2 --- Other photosynthetic lineages --- p.52 / Chapter 4.1.2.3 --- Other non-photosynthetic lineages --- p.55 / Chapter 4.1.2.4 --- Novel higher-level lineages --- p.56 / Chapter 4.2. --- Spatial and seasonal variations of picoeukaryotes --- p.58 / Chapter 4.2.1 --- Spatial variations --- p.59 / Chapter 4.2.1.1 --- Compositional variations --- p.60 / Chapter 4.2.1.2 --- Variations in diversity --- p.61 / Chapter 4.2.2 --- Seasonal variations --- p.65 / Chapter 4.2.2.1 --- Compositional variations --- p.65 / Chapter 4.2.2.2 --- Variations in diversity --- p.66 / Chapter 4.3. --- Methodological aspects --- p.67 / Chapter 4.3.1 --- Sample collection --- p.67 / Chapter 4.3.2 --- PCR amplification --- p.68 / Chapter 4.3.3 --- Cloning and RFLP screening --- p.69 / Chapter 4.3.4 --- Statistical estimates --- p.71 / Chapter 4.3.5 --- Future directions --- p.71 / Chapter Chapter 5. --- General conclusion --- p.73 / References --- p.81

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_326037
Date January 2007
ContributorsCheung, Man Kit., Chinese University of Hong Kong Graduate School. Division of Biology.
Source SetsThe Chinese University of Hong Kong
LanguageEnglish, Chinese
Detected LanguageEnglish
TypeText, bibliography
Formatprint, xii, 93 leaves : ill. ; 30 cm.
CoverageChina, Hong Kong, China, Hong Kong, Hong Kong, Hong Kong, Tolo Harbor (Hong Kong, China), Mirs Bay (China), Tolo Harbor (Hong Kong, China), Mirs Bay (China)
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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