The increase in the prevalence of obesity and obesity-related diseases has caused greater attention to be placed on the molecular mechanisms controlling adipogenesis. In this study, we studied the role of 5-aza-2'-deoxycytidine (5-Aza-dC), an inhibitor of DNA methylation, on adipocyte differentiation. We found that inhibiting DNA methylation by 5-Aza-dC significantly inhibited adipocyte differentiation whereas promoting osteoblastogenesis. Wnt10a was up-regulated by 5-Aza-dC treatment and it was suggested that Wnt10a might play a vital role in suppressing adipogenesis and promoting osteoblastogenesis by inhibiting DNA methylation. In 3T3-L1 cells, Wnt signaling inhibitor IWP-2 was found to reverse the inhibitory effect of 5-Aza-dC on Adipocyte differentiation, whereas in mesenchymal stem cell line, ST2 cells, IWP-2 treatment reversed the effect of 5-Aza-dC on promoting osteoblastogenesis. These studies will provide a better understanding to the cause and treatment of obesity and bone-related diseases.
| Identifer | oai:union.ndltd.org:GEORGIA/oai:scholarworks.gsu.edu:biology_theses-1058 |
| Date | 12 August 2014 |
| Creators | Chen, Yii-Shyuan |
| Publisher | ScholarWorks @ Georgia State University |
| Source Sets | Georgia State University |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Biology Theses |
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