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Previous issue date: 2013-03-11 / Sulfated polysaccharides comprise a complex group of macromolecules with a
range of several biological activities, including antiviral activity, anticoagulant,
antiproliferative, antiherp?tica, antitumor, anti-inflammatory and antioxidant. These
anionic polymers are widely distributed in tissues of vertebrates, invertebrates and
algae. Seaweeds are the most abundant sources of sulfated polysaccharides in
nature. The green algal sulfated polysaccharides are homo or heteropolysaccharides
comprised of galactose, glucose, arabinose and/or glucuronic acid. They are
described as anticoagulant, anti-inflammatory, antiviral, anti-angiogenic, antitumor
compounds. However, there are few studies about elucidation and evaluation of
biological/pharmacological effects of sulfated polysaccharides obtained from green
algae, for example, there is only one paper reporting the antinociceptive activity of
sulfated polysaccharides of these algae. Therefore this study aimed to obtain sulfated
polysaccharides of green seaweed Codium isthmocladum and evaluates them as
potential antinociceptive agents. Thus, in this study, the total extract of
polysaccharides of green alga C. isthmocladum was obtained by proteolytic
digestion, followed by fractionation resulting in five fractions (F0.3, F0.5, F0.7, F0.9
and F1.2) by sequential precipitation with acetone. Using the test of abdominal
contractions we observed that the fraction F0.9 was the most potent antinociceptive
aompound. F0.9 consists mainly of a sulfated heterogalactana. More specific tests
showed that Fo.9 effect is dose and time dependent, reaching a maximum at 90 after
administration (10 mg / kg of animal). F0.9 is associated with TRPV1 and TRPA1
receptors and inhibits painful sensation in animals. Furthermore, F0.9 inhibits the
migration of lymphocytes induced peritonitis test. On the other hand, stimulates the
release of NO and TNF-?. These results suggest that F0.9 has the potential to be
used as a source of sulfated galactan antinociceptive and anti-inflammatory / Os polissacar?deos sulfatados comp?em um grupo complexo de
macromol?culas com uma gama de importantes atividades biol?gicas, incluindo
atividade antiviral, anticoagulante, antiproliferativa, antiherp?tica, antitumoral, antiinflamat?ria
e antioxidante. Esses pol?meros ani?nicos s?o amplamente distribu?dos
em tecidos de vertebrados, invertebrados e algas. As algas marinhas s?o as fontes
mais abundantes de polissacar?deos sulfatados na natureza. Os polissacar?deos
sulfatados de algas verdes s?o homo ou heteropolissacar?deos compostos por
galactose, glicose, arabinose e/ou ?cido glucur?nico. Para estes pol?meros s?o
descritas atividades como anticoagulante, anti-inflamat?ria, antiviral,
antiangiog?nica, antitumoral. Por?m, ainda h? poucos estudos de elucida??o e
avalia??o de atividades biol?gicas/farmacol?gicas de polissacar?deos sulfatados
obtidos de algas verdes, por exemplo, h? somente um trabalho relatando a atividade
antinociceptiva de polissacar?deos sulfatados destas algas. Portanto este trabalho
teve como objetivo obter polissacar?deos sulfatados da alga verde Codium
isthmocladum e avalia-los como poss?veis agentes antinociceptivos. Assim, no
presente estudo, o extrato total de polissacar?deos da alga verde C. isthmocladum
foi obtido atrav?s de digest?o proteol?tica, seguida de fracionamento resultando em
cinco fra??es (F0.3; F0.5; F0.7; F0.9 e F1.2) por precipita??o sequencial com
acetona. Com o teste de contra??es abdominais observou-se que a fra??o F0.9 foi o
mais potente antinociceptivo. F0.9 ? composta principalmente por uma
heterogalactana sulfatada. Ensaios mais espec?ficos mostraram que o seu efeito ?
dose e tempo dependente, chegando ao m?ximo com 90 ap?s a administra??o (10
mg/kg de animal). F0.9 se associa a receptores TRPV1 e TRPA1 e inibe sensa??o
dolorosa em animais. Al?m disso, F0.9 inibe a migra??o de linf?citos em ensaios de
peritonite induzida. Por outro lado estimula a libera??o de NO e TNF-?. Esses
resultados sugerem que F0.9 tem potencial para ser usada como fonte de galactana
sulfatada antinociceptiva e anti-inflamat?ria
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12619 |
| Date | 11 March 2013 |
| Creators | Cordeiro, Sara Lima |
| Contributors | CPF:76111830449, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799567J8&dataRevisao=null, Leite, Edda Lisboa, CPF:06341462468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783254U6&dataRevisao=null, Farias, Eduardo Henrique Cunha de, CPF:01164378473, http://lattes.cnpq.br/0933304924768138, Rocha, Hugo Alexandre de Oliveira |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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