Single-particle tracking experiments have measured the distribution of dwell times of various DNAbinding
species — including CRISPR-Cas9, TetR, and LacI — diffusing in living cells. The observed
truncated power law distribution has direct and indirect implications.
One direct implication is that the observed dwell time distribution is inconsistent with the Gaussian
distribution of binding energies generally obtained from bioinformatics. Consideration of length scales
of the nucleus and the measurement is essential to understanding the dwell time distribution.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:38264 |
| Date | 06 February 2020 |
| Creators | Saxton, Michael J. |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English, German |
| Detected Language | English |
| Type | info:eu-repo/semantics/acceptedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 24, urn:nbn:de:bsz:15-qucosa2-378382, qucosa:37838 |
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