Objetivo: Evaluar el efecto antibacteriano in vitro del extracto metanólico del Lepidium meyenii en sus tres ecotipos (maca roja, amarilla y negra) sobre cepas de Streptococcus mutans (ATCC 25175) y Streptococcus sanguinis (ATCC 10556).
Materiales y Métodos: Este estudio in vitro utilizó pocillos embebecidos del extracto metanólico del Lepidium meyenii en sus tres ecotipos colocados en 7 placas petri que contenían cepas de cultivos. La Concentración mínima inhibitoria (CMI) fue evaluada por la técnica de Kirby Bauer y la concentración mínima bactericida (CMB) mediante el Recuento de Unidades Formadoras de Colonia (UFC). Los halos de inhibición se midieron en mm y se registraron mediante estadística descriptiva media y desviación estándar.
Resultados: Frente al Streptococcus mutans (ATCC 25175), la maca roja tuvo una media (+- d.e) de 13.5 ± 0 .60 mm, MIC de 125.0μg/ml y MBC de 62.5 μg/ml; la maca negra 13.3 ± 0.53 mm, 125.0μg/ml y 62.5 μg/ml, respectivamente; y, la amarilla, 12 ± 0.27 mm, 250.0 μg/ml y 125.0 μg/ml, respectivamente. Frente al Streptococcus sanguinis (ATCC 10556), la media (+- d.e) de la maca roja fue 17.6 ± 0.23 mm, MIC de 62.5μg/ml y MBC de 31.3 μg/ml; la maca negra 19.6 ± 0.44 mm, 62.5μg/ml y 31.3 μg/ml, respectivamente; y, la amarilla 14.8 ± 0.26mm, 31.3 μg/ml y 15.6 μg/ml, respectivamente.
Conclusión: Existe efecto antibacteriano del extracto metanólico de Lepidium meyenii en sus tres ecotipos sobre los microorganismos Streptococcus mutans (ATCC 25175) y Streptococcus sanguinis (ATCC 10556). / Objective: To evaluate the in vitro antibacterial effect of methanolic extract of Lepidium meyenii in its three ecotypes (red, yellow and black maca) against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556).
Materials and Methods: This in vitro study used wells embedded in methanolic extract of Lepidium meyenii in its three ecotypes, placed on 7 petri discs containing the bacteria samples. The Minimum inhibitory concentration (MIC) was evaluated using the Kirby Bauer technique and the minimum bactericidal concentration (MBC) using the colony forming unit (CFU). Inhibition halos were measured in mm and analyzed using descriptive statistics and standard deviation.
Results: Against Streptococcus mutans (ATCC 25175), the red maca had a mean result (±sd) of 13.5±0.60 mm, 125.0ug/ml MIC and 62.5ug/ml MBC; the black maca was 13.3±0.53 mm, 125.0ug/ml MIC and 62.5 ug/ml MBC; and the yellow maca was 12±0.27 mm, 250.0 ug/ml and 125.0 ug/ml, respectively. Against Streptococcus sanguinis (ATCC 10556), the mean (±d.e) of the red maca was 17.6±0.23 mm, 62.5ug/ml MIC and 31.3 ug/ml MBC; the black maca was 19.6±0.44 mm, 62.5ug/ml MIC and 31.3 ug/ml MBC, and the yellow maca was 14.8±0.26 mm, 31.3 ug/ml and 15.6 ug/ml, respectively.
Conclusion: Exists an antibacterial effect of methanolic extract of Lepidium meyenii in its three ecotypes against Streptococcus mutans (ATCC 25175) and Streptococcus sanguinis (ATCC 10556). / Tesis
| Identifer | oai:union.ndltd.org:PERUUPC/oai:repositorioacademico.upc.edu.pe:10757/654707 |
| Date | 29 October 2020 |
| Creators | Alvitez Jurado, Maricielo, Cardenas Cabanillas, Veronica Lucia |
| Contributors | Del Valle Mendoza, Juana Mercedes |
| Publisher | Universidad Peruana de Ciencias Aplicadas (UPC), PE |
| Source Sets | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Language | Spanish |
| Detected Language | English |
| Type | info:eu-repo/semantics/bachelorThesis |
| Format | application/pdf, application/epub, application/msword |
| Source | Universidad Peruana de Ciencias Aplicadas (UPC), Repositorio Académico - UPC |
| Rights | info:eu-repo/semantics/openAccess, Attribution-NonCommercial-ShareAlike 4.0 International, http://creativecommons.org/licenses/by-nc-sa/4.0/ |
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