The recent upsurge of multidrug resistant bacteria (MDRB) among global communities has
become one of the most serious challenges facing health professionals and the human population
worldwide. Cationic ultrashort antimicrobial peptides (USAMPs) are a promising group of molecules
that meet the required criteria of novel antimicrobial drug development. UP-5, a novel penta-peptide,
displayed significant antimicrobial activities against various standard and clinical isolates of MDRB.
UP-5 displayed MICs values within the range of (10–15 M) and (55–65 M) against Gram-positive
and Gram-negative bacteria, respectively. Furthermore, UP-5 displayed antibiofilm activity with
minimum biofilm eradication concentration (MBEC) value as equal to twofold higher than MIC value.
At the same inhibitory concentrations, UP-5 exhibited very low or negligible toxicity toward human
erythrocytes and mammalian cells. Combining UP-5 with conventional antibiotics led to a synergistic
or additive mode of action that resulted in the reduction of the MIC values for some of the antibiotics
by 99.7% along a significant drop in MIC values of the peptide. The stability profile of UP-5 was
evaluated in full mouse plasma and serum with results indicating a more stable pattern in plasma.
The present study indicates that USAMPs are promising antimicrobial agents that can avoid the
negative characteristics of conventional antimicrobial peptides. Additionally, USAMPs exhibit good
to moderate activity against MDRB, negligible toxicity, and synergistic outcomes in combination with
conventional antimicrobial agents.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84601 |
| Date | 06 April 2023 |
| Creators | Almaaytah, Ammar, Qaoud, Mohammed T., Mohammed, Gubran Khalil, Abualhaijaa, Ahmad, Knappe, Daniel, Hoffmann, Ralf, Al-Balas, Qosay |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1424-8247, 3 |
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