Hung Sau Ling. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 131-137). / Table of Contents --- p.1 / Acknowledgements --- p.V / Abbreviations --- p.VI / Aim of investigation --- p.IX / Abstract --- p.XI / Chapter Chapter 1 --- General Introduction --- p.1 / Chapter 1.1 --- Cancer Chemotherapy --- p.2 / Chapter 1.2 --- Plants as sources of useful drugs --- p.4 / Chapter 1.3 --- Potent antitumor compounds found in Goniothalamus giganteus --- p.7 / Chapter 1.4 --- Brief introduction of GONIOTRIOL --- p.8 / Chapter 1.5 --- The study on the antitumor activities of the antitumor compounds --- p.9 / Chapter 1.6 --- Biochemistry study of the anticancer agents --- p.10 / Chapter Chapter 2 --- Materials and Methods --- p.18 / Chapter 2.1 --- Materials --- p.19 / Chapter 2.1.1 --- Animals --- p.19 / Chapter 2.1.2 --- "Buffers, Culture Media and Chemicals" --- p.19 / Chapter 2.1.3 --- Cell lines --- p.20 / Chapter 2.1.4 --- Dye solutions --- p.21 / Chapter 2.1.5 --- Reagents and buffers for Agarose gel --- p.21 / Chapter 2.1.6 --- Synthetic goniotriol and its derivatives --- p.21 / Chapter 2.2 --- Methods --- p.23 / Chapter 2.2.1 --- Radioactive Precursor Incorporation Assays --- p.23 / Chapter 2.2.2 --- MTT assay --- p.24 / Chapter 2.2.3 --- Neutral Red assay --- p.24 / Chapter 2.2.4 --- Isolation and preparation of cells --- p.25 / Chapter 2.2.5 --- Assay for the solvent effect --- p.25 / Chapter 2.2.6 --- Assay for the in vitro antitumor activity THC88 on different cell lines --- p.27 / Chapter 2.2.7 --- Assay of the effect of THC86 on solid sarcoma Scl80 in vivo --- p.28 / Chapter 2.2.8 --- Assay of the effect of THC86 on peritoneal Scl80 in vivo --- p.28 / Chapter 2.2.9 --- Assay of the effect of THC89 on peritoneal EAT in vivo --- p.28 / Chapter 2.2.10 --- Assay of synthetic compound (THC89 and THC87) on the mitogenic activity of spleen lymphocytes --- p.29 / Chapter 2.2.11 --- Assay of synthetic compound (THC87) on the proliferation of murine bone marrow cells from compound- treated mice --- p.30 / Chapter 2.2.12 --- "Assay of synthetic compounds (Ml, P51 and P1) on nonmalignant cell-line" --- p.31 / Chapter 2.2.13 --- Assay of antitumor activity of synthetic compound (THC86)on PU5-1.8 --- p.31 / Chapter 2.2.14 --- Assay of the cytocidal effect of THC86 --- p.32 / Chapter 2.2.15 --- "Assay on the effect of THC86 on the synthesis of DNA, RNA and protein" --- p.32 / Chapter 2.2.16 --- Direct DNA cleavage by THC86 --- p.33 / Chapter 2.2.17 --- DNA fragmentation assay / Chapter 2.2.18 --- Assay of the effect of the synthetic compound (THC86) on different growth fraction of the cells / Chapter 2.2.19 --- Mitosis Study / Chapter 2.2.20 --- Assay for the stability of the synthetic compounds / Chapter Chapter 3 --- Structure / activity relationship of the synthetic compounds --- p.36 / Chapter 3.1 --- Results --- p.37 / Chapter 3.1.1 --- In vitro antitumor activity of the synthetic compounds --- p.37 / Chapter 3.2 --- Discussion --- p.45 / Chapter Chapter 4 --- Antitumor activities of the synthetic compounds --- p.63 / Chapter 4.1 --- Results --- p.64 / Chapter 4.1.1 --- Solvent effect in the screening process --- p.64 / Chapter 4.1.2 --- The effect of the synthetic compound (THC88) on different cell lines --- p.69 / Chapter 4.1.3 --- In vivo anti-tumor activities of the synthetic compounds --- p.71 / Chapter 4.1.3a --- Effect of THC86 on solid sarcoma Sc180 in vivo --- p.71 / Chapter 4.1.3b --- Effect of THC86 on peritoneal Scl80 in vivo --- p.71 / Chapter 4.1.3c --- Effect of THC89 on peritoneal EAT in vivo --- p.72 / Chapter 4.1.4 --- Cytotoxic effect of the tested compounds on normal cells --- p.77 / Chapter 4.1.4a --- Cytotoxic effect of THC89 on normal splenocytes in vitro --- p.77 / Chapter 4.1.4b --- Effect of THC87 on the proliferation of splenocytes --- p.77 / Chapter 4.1.4c --- Effect of THC87 on the proliferation of murine bone marrow cells --- p.78 / Chapter 4.1.4d --- Cytotoxic effect on non-malignant cell-line BALB/c 3T3/A31 --- p.78 / Chapter 4.2 --- Discussion --- p.85 / Chapter Chapter 5 --- The study on the antiproliferative mechanisms of the synthetic compounds --- p.88 / Chapter 5.1 --- Results --- p.89 / Chapter 5.1.1 --- "Effect of the synthetic compounds on Cell Growth, DNA, RNA and Protein" --- p.89 / Chapter 5.1.1a --- Effect of THC86 on PU5-1.8 (macrophage-like tumor) --- p.89 / Chapter 5.1.1b --- Cytocidal effect of THC86 on EAT --- p.89 / Chapter 5.1.1c --- "Effect of the synthetic compounds on synthesis of DNA, RNA and protein" --- p.90 / Chapter 5.1.2 --- Study of the synthetic compounds on the interactions of DNA --- p.101 / Chapter 5.1.2a --- DNA cleavage assay --- p.101 / Chapter 5.1.2b --- DNA fragmentation assay --- p.101 / Chapter 5.1.3 --- Effect of the synthetic compounds on different growth fraction of the cells --- p.104 / Chapter 5.1.4 --- Mitosis study of the synthetic compounds --- p.106 / Chapter 5.1.5 --- Investigation of the stability of the synthetic compounds in culture medium --- p.112 / Chapter 5.2 --- Discussion --- p.117 / Chapter Chapter 6 --- General Discussion --- p.122 / References --- p.131
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318202 |
| Date | January 1994 |
| Contributors | Hung, Sau Ling., Chinese University of Hong Kong Graduate School. Division of Biochemistry. |
| Publisher | Chinese University of Hong Kong |
| Source Sets | The Chinese University of Hong Kong |
| Language | English |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 137 leaves : ill. (some mounted col.) ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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