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Previous issue date: 2017-03-20 / Introduction: Peritonitis remains a major complication in peritoneal dialysis patients, with the predominant infectious agent being coagulase-negative Staphylococcus (CNS). Intraperitoneal administration of antibiotics is the required treatment, however, no consensus exists on the appropriate disinfection of the medication port (MP). Objective: To compare different disinfection techniques for the peritoneal dialysis bag MP. Methods: An experimental study was conducted testing different cleaning agents (70% alcohol vs 2% chlorhexidine) and time periods (5, 10 and 60seconds) for disinfection of the MP. Four microorganisms (S. aureus, E.coli, A. baumannii and C.parapsilosis) were prepared for use as contaminants of the MP. MP were incubated in Tryptic soybroth at 36?C for 24 h, after which, they were seeded on a Biom?rieux? blood agar plate and incubated for 24 h at 36?C. Results: A total of 240 PD bags were contaminated with four different microorganisms. Two positive cultures (E. coli and S. aureus) were identified, both after disinfection with alcohol after 5 and 10 seconds of friction, and none in the chlorhexidine group.
Conclusion: although there was no statistical difference between the antiseptics used and the cleaning time, the use of chlorhexidine for 1 minute was the only one in which there was no bacterial growth, therefore we considered this recommendation. / Introdu??o: A Di?lise Peritoneal utiliza a membrana peritoneal para realizar trocas entre o sangue e a solu??o de di?lise. Peritonite ? a maior complica??o, e o germe predominante ? o Staphylococcus coagulase negativa (SCN). O tratamento para peritonite ? realizado com a administra??o de antibi?tico intraperitoneal. Durante o treinamento, o paciente e/ou familiar s?o capacitados a administrar antibi?tico via intraperitoneal, se necess?rio. No entanto, n?o existe consenso quanto ? maneira mais apropriada para a desinfec??o do tubo entrada de medica??o (TEM). Empresas que comercializam o material de DP recomendam que, para a administra??o de antibi?ticos e outros medicamentos, o TEM deve ser limpo durante cinco minutos com ?lcool a 70%, iodo povidine alc?olico ou clorexidina alco?lica. Objetivo: Comparar a efic?cia das t?cnicas e produtos de desinfec??o do TEM da bolsa de di?lise peritoneal. M?todo: Foi realizado um estudo experimental com diferentes agentes de limpeza (?lcool x clorexidina 2%) e per?odos de tempo (5, 10, 60 segundos) para a desinfec??o do TEM. Foram preparados quatro micro-organismos (S. aureus, E. coli, A. baumanni e C. parapsilosis) para utiliza??o como contaminantes e foram incubados em Caldo de Soja Tripticase?na a 36? C durante 24 horas, ap?s foram semeados por deple??o em placas de ?gar sangue (AS) e incubados durante 24 horas a 36? C. Resultados: No total, 240 bolsas PD foram contaminadas com quatro micro-organismos diferentes. Foram identificadas duas culturas positivas (E. coli e S. aureus), ambas ap?s desinfec??o com ?lcool 70% com o tempo de 5 e 10 segundos de atrito. Conclus?o: Embora sem diferen?a estat?stica entre os antiss?pticos utilizados e o tempo de limpeza, o uso de clorexidine por 1 minuto foi o ?nico em que n?o houve crescimento bacteriano, portanto achamos pertinente esta recomenda??o.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/7646 |
| Date | 20 March 2017 |
| Creators | Conti, Adriana |
| Contributors | Figueiredo, Ana Elizabeth Prado Lima |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, Brasil, Escola de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 500, 500, 500, -224747486637135387, -969369452308786627 |
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