ABBREVIATION
E – strength of electric field
τ – pulse duration
HV – high voltage, short pulses
LV – low voltage, long pulses
MEM - Minimum Essential Medium
S – MEM - Spinner Minimum Essential medium
LISPB – low ionic strength pulsing buffer
ECT – electrochemotherapy
LLC – Lewis Lung Carcinoma
DC-3F – Chinese hamster lung fibroblastocites
LPB – fibrosarkoma cell line
BLM – bleomycin
CPA – cyclophosphamide
LY – Lucifer Yellow
1. INTRODUCTION
The delivery of appropriate short and intense electric pulses to living cells, either in suspension or in tissue, results in a transient and reversible alteration of their cell membrane [Mir et al., 1995; Neumann et al., 1989]. The concept widely accepted is that under influence of strong external electric field the potential difference on the cell membrane occurs [Bernhardt and Pauly, 1973]. As a result of it the nanosize transient aqueous pores in the plasma membrane are created [Abidor et al, 1979, Leikin et al., 1986]. The process is called electroporation and initiated pores are named electropores [Neumann et al., 1989]. It is proposed that at higher field strength and pulse duration more and larger pores are initiated [Glaser et al., 1988]. At the tolerant pulse intensities the resealing of pores takes place and membrane came back to the previous state. The process is called reversible electroporation - REP [Weaver and Chizmadzhev, 1966]. Resealing of pores is in a range of milliseconds - minutes and often is... [to full text]
| Identifer | oai:union.ndltd.org:LABT_ETD/oai:elaba.lt:LT-eLABa-0001:E.02~2006~D_20060913_145004-24823 |
| Date | 13 September 2006 |
| Creators | Šalomskaitė-Davalgienė, Sonata |
| Contributors | Sederevičius, Antanas, Griciūtė, Liudvika-Laima, Saulis, Gintautas, Skeberdis, Vytenis Arvydas, Kriščiukaitis, Algimantas, Alaburda, Aidas, Jurevičius, Jonas, Kaunas University of Medicine |
| Publisher | Lithuanian Academic Libraries Network (LABT), Kaunas University of Medicine |
| Source Sets | Lithuanian ETD submission system |
| Language | Lithuanian |
| Detected Language | English |
| Type | Doctoral thesis |
| Format | application/pdf |
| Source | http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20060913_145004-24823 |
| Rights | Unrestricted |
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