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Previous issue date: 2008-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Blastocystis hominis is one of the most prevalent parasites of the human gastrointestinal tract, but its pathogenic role still remains uncertain. Some organisms similar to B. hominis have been described in a range of non-human hosts and are denominated Blastocystis sp., but their zoonotic potential still needs clarification. The objective of this work was to investigate the occurrence of natural infection by Blastocystis sp. in three species of domesticated birds sold at cooperative markets (with individual vendor stalls) in two districts of the city of Rio de Janeiro and to characterize the morphology of these organisms found in bird droppings, to enable a precise diagnosis. A total of 214 birds were purchased: 35 ducks (Anas platyrhynchos) from market A and 38 from market B, 35 quails (Coturnix japonica) from market A and 36 from market B and 35 chicks (Gallus gallus) from market A and 35 from market B. Of the birds studied from market A, 8 chicks (22.9%), 15 quails (42.9%) and 13 ducks (37.1%) presented biological forms of Blastocystys sp. in their droppings, while at the market B, the respective figures were 15 chicks (42.9%), 2 quails (5.56%) and 21 ducks (55.3%). There was no statistically significant difference in relation to the occurrence of infection in ducks and chicks between the two markets studied, but there was between the quails at the two markets. Considering the number of infected birds at the two markets irrespective of species, the difference was statistically significant. From the observations of the stained fecal smears of the animals that were positive for Blastocystis sp., it was possible to characterize six forms of organisms, namely vacuolar, granular, ameboid, cystic, avacuolar and multivacuolar. It was also possible to characterize the reproductive forms of Blastocystis sp. as binary division, endodyogeny, budding, plamotomy and schizogeny. Even though there are no studies confirming the zoonotic and pathogenic potential of Blastocystis sp., either to human or animal hosts, we suggest that environmental contamination can be occurring at these markets, due to the poor hygienic and sanitary conditions, thus posing a risk to both the animals and the people who circulate there. The knowledge of evaluative forms that were found and characterized in this work is important to gives a precise and faithful diagnosis of Blastocystis sp. in dropping. / Blastocystis hominis ? um dos parasitos de maior preval?ncia no trato gastrintestinal de humanos, por?m seu papel patog?nico ainda permanece incerto. Alguns organismos semelhantes ? B. hominis t?m sido descritos em uma variedade de hospedeiros n?o-humanos sendo denominado Blastocystis sp., mas seu potencial zoon?tico n?o est? esclarecido. O objetivo desse trabalho foi verificar a ocorr?ncia de infec??o natural de Blastocystis sp. em tr?s esp?cies de aves dom?sticas comercializadas em dois mercados municipais do Rio de Janeiro, caracterizando morfologicamente esses organismos encontrados em material fecal para a emiss?o de um diagn?stico preciso. Foram adquiridas 214 aves, sendo 35 patos (Anas platyrhynchos) do mercado A e 38 do mercado B, 35 codornas (Coturnix japonica) do mercado A e 36 do mercado B, e 35 pintos (Gallus gallus)do mercado A e 35 do mercado B. Das aves pesquisadas no mercado A, 8 (22,9%) pintos, 15 (42,9%) codornas e 13 (37,1%) patos apresentaram formas biol?gicas de Blastocystis sp. nas fezes, enquanto que no mercado B, 15 (42,9%) pintos, 2 (5,56%) codornas e 21 (55,3%) patos apresentaram tais formas nas fezes. N?o houve uma diferen?a estatisticamente significativa em rela??o ? ocorr?ncia de infec??o em patos e pintos dos dois mercados municipais pesquisados, ocorrendo essa diferen?a somente entre as codornas dos dois mercados. Ao considerarmos o n?mero de aves infectadas nos dois mercados, independentemente da esp?cie, observou-se que n?o houve uma diferen?a estatisticamente significativa entre os dois mercados. Das observa??es de esfrega?o de fezes coradas com Giemsa daqueles animais que apresentaram positividade para Blastocystis sp., foi poss?vel caracterizar seis formas do organismo, sendo estas: vacuolar, granular, ameb?ide, c?sticas, avacuolar e multivacuolar. Tamb?m foi poss?vel caracterizar as formas reprodutivas de Blastocystis sp. como: divis?o bin?ria, endodiogenia, brotamento, plamatomia e esquizogonia. Mesmo n?o tendo estudos que confirmem o potencial zoon?tico e patog?nico de Blastocystis sp., tanto para hospedeiros humanos quanto para animais, sugere-se que nesses mercados de comercializa??o pode ocorrer a contamina??o ambiental, devido ?s condi??es higi?nicas e sanit?rias serem de p?ssima qualidade, sendo um risco para os animais, assim como para a popula??o humana que comumente freq?entam esses ambientes. O conhecimento das formas evolutivas que foram encontradas e caracterizadas no presente trabalho ? de grande import?ncia para que se tenha um diagn?stico preciso e confi?vel de Blastocystis sp. em material fecal.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:tede/781 |
| Date | 27 February 2008 |
| Creators | Cruz, Wyslaine Costa da |
| Contributors | Bomfim, Teresa Cristina Bergamo do |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Curso de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Parasitologia Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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