Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa.de:bsz:14-qucosa-234947 |
Date | 11 June 2018 |
Creators | Thieme, Sebastian, Holzbaur, Alexander, Wiedemuth, Ralf, Binner, Aline, Navratiel, Katrin, Anastassiadis, Konstantinos, Brenner, Sebastian, Richter, Cornelia |
Contributors | Public Library of Science , |
Publisher | Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden |
Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
Language | English |
Detected Language | English |
Type | doc-type:article |
Format | application/pdf |
Source | PLOS ONE (2018), 13(2). S. 1-10. ISSN: 1932-6203. DOI: 10.1371/journal.pone.0192437. |
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