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The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile

Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.

Identiferoai:union.ndltd.org:DRESDEN/oai:qucosa.de:bsz:14-qucosa-234947
Date11 June 2018
CreatorsThieme, Sebastian, Holzbaur, Alexander, Wiedemuth, Ralf, Binner, Aline, Navratiel, Katrin, Anastassiadis, Konstantinos, Brenner, Sebastian, Richter, Cornelia
ContributorsPublic Library of Science ,
PublisherSaechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden
Source SetsHochschulschriftenserver (HSSS) der SLUB Dresden
LanguageEnglish
Detected LanguageEnglish
Typedoc-type:article
Formatapplication/pdf
SourcePLOS ONE (2018), 13(2). S. 1-10. ISSN: 1932-6203. DOI: 10.1371/journal.pone.0192437.

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