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Previous issue date: 2009-03-13 / Endometriosis it is considered as the presence of ectopic endometrial tissue, with similar
histology and function to the endometrium usually located. However, the explanation for
the implantation of the endometrial tissue in certain women is still unknown. The
determination of the degree of compromising of the endometriosis is based on a system of
points proposed by American Fertility Society finds of the laparoscopy. Endometriosis is a
disease that occurs mainly in women in reproductive age. The disease can be related with
infertility in 30% to 40% of the cases. The possible mechanisms as the endometriosis as
cause of the infertility are: (a) interference with the sexual function (dyspareunia, reduction
of the frequency of sexual intercourse). (b) Interference with the ovulation: (anovulation;
deficient luteal phase and luteinization phase. The aim of this study was to determine the
frequency of p53 polymorphism codon 72, in two groups of patients with endometriosis
and other symptoms of the disease. The study included 38 peripheral blood samples from
women submitted to videolaparoscopy that confirmed endometriosis. The patients were
divided into two groups of 19 women each, one with infertility and the other not. The
polymorphism was evaluated by PCR. The data were submitted to statistical analysis using
the chi-square and odds ratio tests. Of the patients who only had pain and/or bleeding
without infertility, 09 (47.3%) were arginine homozygous and 10 (52.6%) were proline
homozygote or heterozygote. Of those who presented with infertility associated with pain
and/or bleeding, 12 (100%) were proline homozygous or heterozygous, and in those with
only complaint of infertility, 05 (71.4%) were arginine homozygous and 02 (28.6%)
proline homozygous or heterozygous). In correlating pain intensity, 04 (23.5%) women
with the proline allele (homozygous or heterozygous) reported slight or moderate pain and
20 (95.5%) intense pain. All these differences were statistically significant (p= 0.003). In
relating pain intensity to laparoscopic classification, the following was found: 08 (50%)
patients who showed slight or moderate pain had a classification I or II and 08 (50%) III or
IV. Of those with intense pelvic pain, 08 (36.4%) were included in classification I or II and
14 (63.6%) in III or IV. The proline allele (homozygous or heterozygous) is linked more to
patients with infertility and with a more severe clinical picture. / A endometriose é conceituada como a presença de tecido endometrial ectópico, com
histologia e função semelhante ao endométrio normalmente situado. No entanto, a
explicação para a implantação do tecido endometrial em determinadas mulheres ainda é
desconhecida. O grau do comprometimento da endometriose baseia-se num sistema de
pontos proposta pela American Fertility Society (1978), hoje denominada American
Society for Reproductive Medicine com base nos achados de laparoscópica. A intenção
deste estudo foi determinar a freqüência do polimorfismo do p53, códon 72, em dois
grupos de pacientes com endometriose e outros sintomas da doença. O estudo incluiu 38
amostras de sangue periférico de mulheres submetidas à videolaparoscopia com
diagnóstico confirmado de endometriose. As pacientes foram divididas em dois grupos de
19 mulheres cada, um com infertilidade e o outro não. O polimorfismo foi avaliado por
PCR. Os dados foram submetidos a análise estatística sendo usado o teste de qui-quadrado
e Odds Ratio. Dos pacientes que só tiveram dor e/ou sangramento sem infertilidade,
09(47,3 %) eram arginina homozigoto e 10(52,6%) eram prolina homozigoto ou
heterozigoto. Das pacientes que apresentaram infertilidade associado à dor e/ou
sangramento 12(100%) era prolina homozigoto ou heterozigoto. Das pacientes que
queixavam apenas de infertilidade, 05 (71,4%) eram arginina homozigoto e 02 (28,6%)
prolina homozigoto ou heterozigoto. Correlacionando intensidade da dor, 04(23,5%) com
alelo prolina (homozigoto ou heterozigoto) informaram dor leve e/ou moderada e
20(95,5%) dor intensas. Todas essas diferenças foram estatisticamente significativas
(p=0,003). Em intensidade da dor relacionado com a classificação laparoscópica foi
encontrado o seguinte: 08(50%) pacientes que tiveram dor leve e/ou moderada tiveram
classificação I ou II e 08(36,4%) foram incluídos na classificação III ou IV. Das pacientes
com dor intensa, 08(36,4%) foram incluídas na classificação I ou II e 14 (63,6%) em III ou
IV. Conclui-se que a presença do alelo prolina (homozigoto ou heterozigoto) está mais
relacionado com pacientes com endometriose e com quadro clínico mais severo da doença.
| Identifer | oai:union.ndltd.org:IBICT/oai:ambar:tede/2419 |
| Date | 13 March 2009 |
| Creators | Ribeiro Júnior, Circoncisto Laurentino |
| Contributors | Moura, Katia Karina Verolli de Oliveira |
| Publisher | Pontifícia Universidade Católica de Goiás, Genética, PUC Goiás, BR, Ciências Humanas |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_GOAIS, instname:Pontifícia Universidade Católica de Goiás, instacron:PUC_GO |
| Rights | info:eu-repo/semantics/openAccess |
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