Orientador: Diogo Teruo Hashimoto / Resumo: The pirapitinga, Piaractus brachypomus (Characiformes, Serrasalmidae), is a fish that occurrs in the Amazon basin and it is considered as one of the main native species used for production in aquaculture in South America. The main objectives of this study were: 1) to perform the transcriptome sequencing of P. brachypomus through Next Generation Sequencing (NGS) and then characterize a set of microsatellite markers for this species; 2) to apply microsatellite polymorphic markers for analysis of genetic variability in cultured stocks of pirapitinga. The transcriptome sequencing was carried out through the Roche/454 technology, which resulted in 3,696 non-redundant (nr) contigs. Of these total, 2,568 genes had similarity in the protein database nr (Genbank) and were characterized in the categories of Gene Ontology (GO), with 2,075 sequences (80.8%) annotated in GO terms. After the validation process of 30 microsatellite loci, 8 microsatellite markers showed polymorphism. The analysis of these polymorphic markers in cultured stocks revealed that the Northern fish farms had a higher genetic diversity (allele richness and heterozygosity) than the Southeastern fish farms. In addition, the AMOVA results demonstrated the highest variation present within the populations (62.5%). However, when comparing the groups according to the geographic distribution (Wild, North Fish farms and fish farms in the Southeast), it was observed a considerable variation (31.76%) among the groups. The Fst ... (Resumo completo, clicar acesso eletrônico abaixo) / Mestre
| Identifer | oai:union.ndltd.org:UNESP/oai:www.athena.biblioteca.unesp.br:UEP01-000872842 |
| Date | January 2016 |
| Creators | Jorge, Paulo Henrique |
| Contributors | Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Botucatu). |
| Publisher | Botucatu, |
| Source Sets | Universidade Estadual Paulista |
| Language | Portuguese |
| Detected Language | English |
| Type | computer file |
| Relation | Sistema requerido: Adobe Acrobat Reader |
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