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The Measurement of erythrocyte carbonic anhydrase and its use in the diagnosis of thyrotoxicosis.

by Judy, Po-shan Lai. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references(leaves 83-87). / Abbreviations --- p.iii / List of Tables --- p.iv / List of Figures --- p.v / Acknowledgements --- p.vi / SUMMARY --- p.1 / Chapter 1. --- INTRODUCTION --- p.2 / Chapter 2. --- BACKGROUND --- p.4 / Chapter 2.1 --- PROBLEMS ENCOUNTERED IN THYROID FUNCTION TESTS --- p.4 / Chapter 2.2 --- A NEED FOR TISSUE MARKER VERSUS EXISTING THYROID FUNCTION TESTS --- p.8 / Chapter 2.3 --- CHOICE OF TISSUE MARKER AS AN ADJUNCT TO THYROID FUNCTION TESTS --- p.10 / Chapter 2.3.1 --- ERYTHROCYTE ZINC --- p.10 / Chapter 2.3.2 --- ERYTHROCYTE CARBONIC ANHYDRASE ISOENZYME --- p.11 / Chapter 3. --- CARBONIC ANHYDRASE --- p.14 / Chapter 3.1 --- HISTORY AND BACKGROUND --- p.14 / Chapter 3.2 --- CARBONIC ANHYDRASE ISOENZYMES --- p.15 / Chapter 3.2.1 --- STRUCTURE AND DISTRIBUTION --- p.15 / Chapter 3.2.2 --- GENERAL MECHANISMS --- p.18 / Chapter 3.2.3 --- GENETICS AND REGULATION --- p.18 / Chapter 3.2.4 --- PHYSIOLOGICAL AND CLINICAL ASPECTS --- p.20 / Chapter 4. --- ANALYTICAL METHODS FOR MEASUREMENT OF ERYTHROCYTE CARBONIC ANHYDRASE --- p.22 / Chapter 4.1 --- DETERMINATION OF ECAI USING IMMUNOTURBIDIMETRIC ASSAY --- p.23 / Chapter 4.1.1 --- PRINCIPLE OF TECHNIQUE --- p.23 / Chapter 4.1.2 --- COLLECTION AND HANDLING OF BLOOD SPECIMENS --- p.29 / Chapter 4.1.3 --- PREPARATION OF HAEMOLYSATE --- p.31 / Chapter 4.1.4 --- MEASUREMENT OF HAEMOLYSATE CAI --- p.32 / Chapter 4.1.5 --- OPTIMIZATION PROCEDURE --- p.34 / Chapter 4.1.6 --- EVALUATION EXPERIMENTS --- p.36 / Chapter 4.1.7 --- DETERMINATION OF HAEMOGLOBIN CONCENTRATION IN HAEMOLYSATE --- p.39 / Chapter 4.1.8 --- DETERMINATION OF MEAN CELL HAEMOGLOBIN CONCENTRATION --- p.41 / Chapter 4.1.9 --- CALCULATION OF ERYTHROCYTE CARBONIC ANHYDRASE I CONCENTRATION --- p.43 / Chapter 4.2 --- DETERMINATION OF ECAI USING RADIAL IMMUNODIFFUSION METHOD --- p.43 / Chapter 5. --- RESULTS OF OPTIMIZATION AND EVALUATION EXPERIMENTS --- p.46 / Chapter 5.1 --- OPTIMIZATION RESULTS --- p.46 / Chapter 5.1.1 --- WAVELENGTH --- p.46 / Chapter 5.1.2 --- PEG CONCENTRATION --- p.49 / Chapter 5.1.3 --- SAMPLE VOLUME --- p.52 / Chapter 5.1.4 --- ANTIBODY DILUTION --- p.52 / Chapter 5.1.5 --- TEMPERATURE --- p.54 / Chapter 5.1.6 --- TIME OF INCUBATION --- p.54 / Chapter 5.1.7 --- OPTIMIZED TEST PROTOCOL --- p.56 / Chapter 5.2 --- EVALUATION RESULTS --- p.56 / Chapter 5.2.1 --- STABILITY --- p.56 / Chapter 5.2.2 --- LINEARITY --- p.58 / Chapter 5.2.3 --- PRECISION --- p.62 / Chapter 5.2.3.1 --- WITHIN-RUN --- p.62 / Chapter 5.2.3.2. --- BETWEEN-RUN --- p.62 / Chapter 5.2.4 --- CROSS-REACTIVITY --- p.62 / Chapter 5.2.5 --- RECOVERY --- p.62 / Chapter 5.2.6 --- COMPARISON WITH COMPARATIVE METHOD --- p.64 / Chapter 5.3 --- DISCUSSION --- p.64 / Chapter 6. --- ECAI IN NORMAL SUBJECTS --- p.67 / Chapter 6.1 --- SUBJECTS AND METHOD --- p.67 / Chapter 6.2 --- RESULTS --- p.67 / Chapter 6.3 --- DISCUSSION --- p.67 / Chapter 7. --- PATIENT STUDY --- p.69 / Chapter 7.1 --- SUBJECTS AND METHOD --- p.69 / Chapter 7.2 --- RESULTS --- p.70 / Chapter 7.2.1 --- DEMOGRAPHIC AND BIOCHEMICAL PARAMETERS --- p.70 / Chapter 7.2.2 --- CORRELATION BETWEEN ECAI AND FT3 RESULTS OF THYROTOXIC PATIENTS BEFORE TREATMENT --- p.71 / Chapter 7.2.3 --- CORRELATION BETWEEN ECAI AND EZN RESULTS OF THYROTOXIC PATIENTS --- p.71 / Chapter 7.2.4 --- RELATIONSHIP BETWEEN THE CHANGES IN ECAI AND FT3 RESULTS IN THYROTOXIC PATIENT ON TREATMENT --- p.71 / Chapter 7.3 --- DISCUSSION --- p.76 / Chapter 8. --- GENERAL DISCUSSION --- p.79 / Chapter 9. --- REFERENCES --- p.83

Identiferoai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_318917
Date January 1992
ContributorsLai, Po-shan., Chinese University of Hong Kong Graduate School. Division of Clinical and Pathological Sciences.
PublisherChinese University of Hong Kong
Source SetsThe Chinese University of Hong Kong
LanguageEnglish
Detected LanguageEnglish
TypeText, bibliography
Formatprint, vi, 87 leaves : ill. ; 30 cm.
RightsUse of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

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