Return to search

Thromboresistant and rapid-endothelialization effects of dopamine and staphylococcal protein A mediated anti-CD34 coating on 316L stainless steel for cardiovascular devices

There is convincing evidence in vivo that the vascular homing of endothelial progenitor cells (EPCs) contributes to rapid endothelial regeneration, which could prevent thrombosis and restenosis of cardiovascular devices. To enhance the EPC homing on cardiovascular devices, immobilization of an EPC capture agent (e.g. an anti-CD34 antibody) on the surface of cardiovascular devices is critical. We describe a way of immobilizing anti-CD34 Ab on 316L Stainless Steel (316L SS). For this, surface modification of 316L SS was performed via self-polymerization of dopamine (DA) and covalent grafting of staphylococcal protein A (SPA). On this coating the anti-CD34 Abs were oriented immobilized through their Fc constant region with SPA. In this process, the results of quartz crystal microbalance, X-ray photoelectron spectroscopy and water contact angle studies indicate that DA, SPA and anti-CD34 Ab were successfully immobilized onto the surface step by step. In vitro blood-compatibility tests confirmed that the modified surface induced less pro-coagulant fibrinogen denaturation, less platelet adhesion and lower activation of the adherent platelets. The affinity of EPCs for the modified surface has been demonstrated under flow conditions. This study provides potential applications for cardiovascular implant materials.

Identiferoai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:36067
Date07 January 2020
CreatorsChen, Jialong, Li, Quanli, Xu, Jianguang, Zhang, Le, Maitz, Manfred F., Li, Jun
PublisherRoyal Society of Chemistry
Source SetsHochschulschriftenserver (HSSS) der SLUB Dresden
LanguageEnglish
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text
Rightsinfo:eu-repo/semantics/openAccess
Relation2050-7518, 10.1039/c4tb01825g, info:eu-repo/grantAgreement/Anhui Medical University/Grants for Scientific Research/XJ201232/

Page generated in 0.0021 seconds