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Previous issue date: 2017-02-22 / CAPES / The parasitism caused by Rhipicephalus microplus, popularly known as cattle tick, causes several losses to the Brazilian livestock. The indiscriminate use of chemical products for its control has generated several negative effects and in this context, studies involving the fungus Metarhizium spp. for biological control of ticks have shown great importance. The present study evaluated the virulence of 11 isolates of Metarhizium spp. for engorged females, eggs and larvae of R. microplus and also the conidiogenesis potential of the same isolates and their relationship with virulence. Single point of inoculum and cultivation between slide and coverslip were performed to evaluate the macro and micromorphology of the isolates. During the bioassay using engorged females, the effects of fungi on reproductive parameters were evaluated by analyzing the control percentage of each isolate after treatment by immersion of females in suspensions of 107 and 108 conidia / mL. The virulence of the isolates for eggs and larvae was evaluated by observing the hatch rate and mortality, respectively, after treatment with aqueous suspensions of Metarhizium spp. at concentrations of 105, 106, 107 and 108 conidia / mL. The LC50 and CL90 of each isolate for larvae were also calculated on the 25th day after treatment. To calculate the conidiogenesis of the isolates, three random cuts of 1.256 cm2 of area of each plate containing fungal conidia of 14 days of culture were made, then stirred in 1 ml of distilled water solution and 0.1% tween 80 and one aliquot of this suspension was quantified using a Neubauer chamber and optical microscope. Parametric data were analyzed by analysis of variance (ANOVA) followed by Tukey test and non-parametric by Kruskal Wallis test followed by Student-Newman-Keuls (SNK) with significance level of 5% through free software R Studio Version 0.99 .903 and the "agricolae" package (Statistical Procedures for Agricultural Research), version 1.2-4. In addition, multivariate statistics were applied using Principal Component Analysis (PCA) for parameters of engorged females. The macro and micromorphological analysis of the isolates tested were compatible with those described in the literature for Metharizium spp. In general, the results were directly proportional to the concentrations that were tested and the isolates ARSEF 729 of Metarhizium anisopliae and ARSEF 3643 of Metarhizium anisopliae senso latu, presented the best results for R. microplus females, eggs and larvae, proving to be good candidates for future studies, whereas the isolate ARSEF 2211 of Metarhizium anisopliae senso latu demonstrated low virulence for all stages. The conidiogenesis of the different isolates tested varied a lot and no relation between conidia production and virulence was observed. The present study made possible the selection of isolates of Metarhizium spp. with high virulence for several stages of life of the R. microplus tick as well as isolates with high conidia production and their relationship with virulence, emphasizing the importance of studies to select suitable isolates for future use in formulations for biological control in the field. / O parasitismo causado por Rhipicephalus microplus, conhecido popularmente como carrapato dos bovinos, acarreta diversos preju?zos para a pecu?ria brasileira. O uso indiscriminado de produtos qu?micos para o seu controle tem gerado diversos efeitos negativos e nesse contexto, estudos envolvendo o fungo Metarhizium spp. para controle biol?gico de carrapatos tem demonstrado grande import?ncia. O presente estudo avaliou a virul?ncia de 11 isolados de Metarhizium spp. para f?meas ingurgitadas, ovos e larvas de R. microplus e tamb?m o potencial de conidiog?nese dos mesmos isolados e a sua rela??o com a virul?ncia. Pontos ?nicos de in?culo e microcultivo entre l?mina e lam?nula foram realizados a fim de avaliar a macro e micro morfologia dos isolados. Durante o bioensaio utilizando f?meas ingurgitadas, foram avaliados os efeitos dos fungos sobre os par?metros reprodutivos atrav?s da an?lise do percentual de controle de cada isolado ap?s o tratamento por imers?o das f?meas em suspens?es aquosas de 107 e 108 con?dios/mL. A virul?ncia dos isolados para ovos e larvas foi avaliada atrav?s da observa??o da taxa de eclos?o e mortalidade, respectivamente, ap?s tratamento com suspens?es aquosas nas concentra??es de 105, 106, 107 e 108 con?dios/mL. Tamb?m foi calculada a CL50 e CL90 de cada isolado para larvas no vig?simo quinto dia ap?s o tratamento. Para calcular a conidiog?nese dos isolados, foram feitos tr?s recortes aleat?rios de 1,256 cm2 de ?rea de cada placa contendo con?dios f?ngicos de 14 dias de cultivo, em seguida foram agitados em 1mL de solu??o de ?gua destilada e tween 80 a 0,1% e uma al?quota desta suspens?o foi quantificada com o auxilio de c?mara de Neubauer e microsc?pio ?ptico. Os dados param?tricos foram avaliados pela an?lise de vari?ncia (ANOVA) seguida do teste de Tukey e os n?o param?tricos pelo teste de Kruskal Wallis seguido de Student-Newman-Keuls (SNK) com n?vel de signific?ncia de 5% atrav?s do software livre R Studio Vers?o 0.99.903 e o pacote ?agricolae? (Statistical Procedures for Agricultural Research), vers?o 1.2-4. Adicionalmente foi aplicada estat?stica multivariada mediante a An?lise de Componentes Principais (ACP) para par?metros de f?meas ingurgitadas. A an?lise macro e micromorfol?gica dos isolados testados se mostrou compat?vel com as descritas na literatura para Metharizium spp. De maneira geral, os resultados se apresentaram diretamente proporcionais ?s concentra??es que foram testadas e os isolados ARSEF 729 de Metarhizium anisopliae e ARSEF 3643 de Metarhizium anisopliae senso latu, apresentaram os melhores resultados para f?meas, ovos e larvas de R. microplus, demonstrando ser bons candidatos para estudos futuros. O isolado ARSEF 2211 de Metarhizium anisopliae senso latu demonstrou baixa virul?ncia para todos os est?gios. A conidiog?nese dos diferentes isolados testados se mostrou muito variada e n?o apresentou nenhuma rela??o com a virul?ncia dos mesmos. O presente estudo possibilitou a sele??o de isolados de Metarhizium spp. com alta virul?ncia para os diferentes est?gios de vida do carrapato R. microplus bem como isolados com alta produ??o de con?dios, ressaltando a import?ncia de estudos que visem selecionar isolados adequados para serem utilizados futuramente em formula??es para controle biol?gico a campo.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2168 |
| Date | 22 February 2017 |
| Creators | JONES, Giselle Arieiro |
| Contributors | Bittencourt, V?nia Rita Elias Pinheiro, Perinotto, Wendell Marcelo de Souza, Bittencourt, V?nia Rita Elias Pinheiro, Angelo, Isabele da Costa, Bezerra, Simone Quinelato |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | ABDEL-SHAFY, S.; SOLIMAN, M. M. M. Toxicity of some essential oils on eggs, larvae and females of Boophilus annulatus (acari: ixodida: amblyommidae) infesting cattle in Egypt. Acarologia, v. 44, p. 23-30, 2004. ALONSO-D?AZ, M.A.; GARCIA, L.; GALINDO-VELASCO, E.; LEZAMA-GUTIERREZ, R.; ANGEL-SAHAGUN, C.A.; RODRIGUEZ-VIVAS, R.I.; FRAGOSO-SANCHEZ, H. Evaluation of Metarhizium anisopliae (Hyphomycetes) for the controlof Boophilus microplus (Acari: Ixodidae) on naturally infested cattle inthe Mexican tropics. Veterinary Parasitology, 147, p. 336?340, 2007. ALVES, S. B. Controle Microbiano de Insetos. Piracicaba, SP: FEALQ, 1998, 1163p. ANDREOTI, R. Situa??o atual da resist?ncia do carrapato-do-boi Rhipicephalus (Boophilus) microplus aos acaricidas no Brasil ? Campo Grande, MS: Embrapa Gado de Corte, 1 edi??o, 2010. 36p. ARRUDA, W; LUBECK, I; SCHRANK, A; VAINSTEIN, M.H. Morphological alterations of Metarhizium anisopliae during penetration of Boophilus microplus ticks. Experimental and Applied Acarology, v. 37, p. 231-244, 2005. AZEVEDO, J.L. Engenharia gen?tica aplicada ao controle microbiano de insetos. In: ALVES, S. B. Controle Microbiano de Insetos. Piracicaba: FEALQ, 1998. p. 239-268. BAHIENSE, T.C.; FERNANDES, E.K.K.; BITTENCOURT, V.R.E.P. Compatibility of the fungus Metarhizium anisopliae and deltamethrin to control a resistant strain of Boophilus microplus tick. Veterinary Parasitology, v. 141, p. 319?324, 2006. BARCI, L.A.G.; ALMEIDA, J.E.M.; NOGUEIRA, A.H.C.; ZAPPELINI, L.O.; PRADO, A.P.; Selection of isolates of entomopathogenic fungus Beauveria bassiana (Ascomycetes: Clavicipitaceae) for control of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Revista Brasileira Parasitologia Veterinaria, v. 18, p.7-13, 2009. BARROS-BATESTI, D. M.; ARZUA, M.; BECHARA, H. G. Carrapatos de import?ncia medico-veterin?ria da regi?o neotropical: um guia ilustrado para identifica??o das esp?cies. 1 ed., S?o Paulo, SP: Butant?, 2006, 223p. BASSO, L.M.D.; MONTEIRO, A.C.; BELO, M.A.A.; SOARES, V.E.; GARCIA, M.V.; MOCHI,D.A. Control of Boophilus microplus larvae by Metarhizium anisopliae in artificially infested pastures. Pesquisa Agropecu?ria Brasileira, 40 (6), p. 595?600, 2005. BENNETT, G. F. Ovoposition of Boophilus microplus (CANESTRINI) (ACARIDA: IXODIDAE) I. Influence of tick size on egg production. Acarologia, v. 16, 1974. BIDOCHKA, M.J.; SMALL, C.L.; SPIRONELLO, M. Recombination within sympatric cryptic species of the insect pathogenic fungus Metarhizium anisopliae. Environmental Microbiology, v.7, p.1361-1368, 2005. BISCHOFF, J.F.; REHNER, S.A.; HUMBER, R.A. Metarhizium frigidum sp. nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia, 98(5), p. 737?745, 2006. BISCHOFF, J.F.; REHNER, S.A.; HUMBER, R.A. A multilocus phylogeny of the Metarhizium anisopliae lineage. Mycologia, v. 101, p. 512-530, 2009. BITTENCOURT, V.R.E.P.; MASSARD, C.L.; LIMA, A.F. A??o do fungo Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1883, em ovos e larvas do carrapato Boophilus microplus (Canestrini, 1887). Revista Universidade Rural ? S?rie Ci?ncias da Vida, v. 16, p. 41-47, 1994. BITTENCOURT, V.R.E.P.; C.L. MASSARD, A.F. LIMA. A??o do fungo Metarhizium anisopliae sobre a fase n?o parasit?ria do ciclo biol?gico de Boophilus microplus. Revista Universidade Rural - S?rie Ci?ncias da Vida, v. 16, p. 49-55, 1994b. BITTENCOURT, V. R. E. P.; MASCARENHAS, A. G.; FACCINI, J. L. H. Mecanismo de infec??o do fungo Metarhizium anisopliae no carrapato Boophilus microplus em condi??es experimentais. Ci?ncia Rural, v. 29, p. 351-354, 1999. BRIDGE, P.D.; PRIOR, C.; SAGBOHAN, J.; LOMER, C.J.; CAREY, M.; BUDDIE, A. Molecular characterization of isolates of Metarhizium from locusts and grasshoppers. Biodiversity and Conservation, v. 6, p. 177-189, 1997. CAMARGO, M.G.; G?LO, P. S.; ANGELO, I.C.; PERINOTTO, W. M. S.; SA, F. A.; QUINELATO, S.; BITTENCOURT, V. R. E. P. Effect of oil-based formulations of acaripathogenic fungi to control Rhipicephalus microplus ticks under laboratory conditions. Veterinary Parasitology, v. 188, p. 140-147, 2012. CAMARGO, M.G.; MARCIANO, A.F.; S?, F.A.; PERINOTTO, W.M.S.; QUINELATO, S.; G?LO, P.S.; ANGELO, I.C.; PRATA, M.C.A.; BITTENCOURT, V.R.E.P. Commercial formulation of Metarhizium anisopliae for thecontrol of Rhipicephalus microplus in a pen study. Veterinary Parasitology 205, p. 271?276, 2014. CHANDLER, D.; DAVIDSON, G.; PELL, J. K.; BALL, B. V.; SHAW, K.; SUNDERLAND, K.D. Fungal biocontrol of Acari. Biocontrol Science and Technology, v. 10, p. 357-384, 2000. CHARNLEY, A. K . Physiological aspects of destructive pathogenesis in insects by fungi: a du Coleoptere Oryctes rhinoceros par le champignon en tomopa thogene Metarhizium anisopliae. Entomophaga 22, p. 289-294, 1984. CHARNLEY, A. K. In Biotechnoiogy of Fungi for improving Plant Growth, ed. J. M. Whipps & R. D. Lumsden. Cambridge University Press, Cambridge, pp. 85-125, 1989. CHARNLEY, A. K. ; ST. LEGER, R. J. In The Fungal Spore and Disease Initiation in Plants and Animals, ed. G. T. Cole & H. C. Hoch. Plenum Press, New York, p. 267-86, 1991. CORREIA, A.C.B.; FIORIN, A.C.; MONTEIRO, A.C.; VER?SSIMO, C.J. Effects of Metarhizium anisopliae on the tick Boophilus microplus (Acari: Ixodidae) in stabled cattle. Journal of Invertebrate Pathology, 71, p. 189?191, 1998. COSTA, G.L.; SARQUIS, M.I.M.; MORAES, A.M.L.; BITTENCOURT, V.R.E.P. Isolation of Beauveria bassiana and Metarhizium anisopliae var. anisopliae from Boophilus microplus tick (Canestrini, 1887), in Rio de Janeiro State, Brazil. Mycopathologia, v. 154, p. 207-209, 2002. D?AZ, M.P.; MAC?AS, A.F.; NAVARRO, S.R.; TORRE, M. Mecanismo de acci?n de los hongos entomopat?genos. Interciencia, v. 31, p. 856-860, 2006. DRIVER, F.; MILNER, R.J. PCR applications to the taxonomy of entomopathogenic fungi. In: BRIDGE, P.D.; ARORA, D.K.; REDDY, C.A.; ELANDER, R.P. Applications in PCR mycology. CABI Publishing, p. 153-186, 1998. DRIVER, F.; MILNER, R.J.; TRUEMAN, J.W.H. A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data. Mycological Research, v. 104, p. 134-150, 2000. DRUMMOND, R. O.; GLADNEY, W. J.; WHETSTONE, T. M.; ERNST, S. E. Laboratory testing of insecticides for control of the winter tick. Journal Economic Entomology, v. 64, p. 686-688, 1971. ECOBICHON D.J. Pesticide use in developing countries. Toxicology; v. 160(1-3): p. 27-33, 2001. FARIA, M. R.; WRAIGHT, S. P. Mycoinsecticides and mycoacaricides: a comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, v. 43, p. 237?256, 2007. FERNANDES, E.K.K.; COSTA, G.L.; SOUZA, E.J.; MORAES, A.M. L.; BITTENCOURT, V.R.E.P. Beauveria bassiana isolated from engorged females and tested against eggs and larvae of Boophilus microplus (Acari: Ixodidae). Journal of Basic Microbiology, v. 43, p. 393-398, 2003. FERNANDES, ?.K.K.; BITTENCOURT, V.R.E.P. Entomopathogenic fungi against South American tick species. Experimental and Applied Acarology, v. 46, p.71-93, 2008. FERNANDES, ?. K. K.; MORAES, ?. M. L.; PACHECO, R. S.; RANGEL, D. E. N.; MILLER, M. P.; BITTENCOURT, V. R. E. P.; ROBERTS, D. W. Genetic diversity among Brazilian isolates of Beauveria bassiana: comparisons with non-Brazilian isolates and other Beauveria species. Journal of Applied Microbiology, v. 107, p. 760-774, 2009. FERNANDES, ?.K.K.; KEYSER, C.A.; CHONG, J.P.; RANGEL, D.E.N.; MILLER, M.P.; ROBERTS, D.W. Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity. Journal of Applied Microbiology, v.108, p.115-128, 2010. FERNANDES, E.K.K.; ANGELO, I.C.; RANGEL, D.E.N.; BAHIENSE,T.C.; MORAES, A. M.L.; ROBERTS, D.W.; BITTENCOURT, V.R.E.P. An intensive search for promising fungal biological control agents of ticks, particularly Rhipicephalus microplus. Veterinary Parasitology, v. 182, p. 307-318, 2011. FERNANDES, E.K.K.; BITTENCOURT, V.R.E.P.; ROBERT, D.W. Perspectives on the potential of entomopathogenic fungi in biological control of ticks. Experimental Parasitology v. 130, p. 300?305. 2012. FRAZZON, A. P. G.; VAZ JUNIOR, I. S.; MASUDA, A.; SCHRANK, A.; VAINSTEIN, M. H. In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus. Veterinary Parasitology, v. 94, p. 117?125, 2000. FREIMOSER F.M.; SCREEN, S.; BAGGA, S.; HU, G.; ST LEGER, R.J. Expresses sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potencial activity in insect hosts. Microbiology, v. 149, p. 239-247, 2003. FURLONG, J. Controle do carrapato dos bovinos na regi?o Sudeste do Brasil. Caderno T?cnico da Escola de Veterin?ria UFMG. v. 8, p. 49-61, 1993. FURLONG, J.; MARTINS, J.R.S. Resist?ncia dos carrapatos aos carrapaticidas. Circular T?cnica n. 59-Centro Nacional de Pesquisa de Gado de Leite. Embrapa Gado de Leite, Juiz de Fora, 2000. 25p. GINSBERG, H. S.; LEBRUN, R. A.; HEYER, K. & ZHIOUA, E. Potential non target effects of Metarhizium anisopliae (Deuteromycetes) used for biological control of ticks (Acari: Ixodidae). Environmental Entomology v. 31, p. 1191?1196, 2002. GOLO, P. S.; GARDNER, D. R.; GRILLEY, M. M.; TAKEMOTO, J. Y.; KRASNOFF, S. B.; PIRES, M. S.; FERNANDES, E. K. K.; BITTENCOURT, V. R. E. P.; ROBERTS, D. W. Production of Dextruxins from Metarhizium spp. Fungi in Artificial Medium and in Endophytically Colonized Cowpea Plants. PLoS ONE v. 9(8): p. 104946, 2014. GONZ?LES, J. C. O carrapato do boi: vida, resist?ncia e controle. S?o Paulo: Mestre Jou, 1974. 101p. GONZALES, J. C. O controle dos carrapatos do boi. 2. Ed. Porto Alegre: Edi??o do Autor, 1995. 79p. GONZ?LES, J.C. O carrapato dos bovinos Boophilus microplus (Can. 1887) (Revis?o hist?rica e conceitual). A Hora Veterin?ria, ano 21, p.23-28, 2002. GRISI, L.; LEITE, R. C.; MARTINS, J. R. S.; BARROS, A. T. M.; ANDREOTTI, R.; CAN?ADO, P. D.; LEON, A. A. P.; PEREIRA, J. B.; VILLELA, H. S. Reassessment of the potencial economic impact of cattle parasites in Brazil. Brazilian Journal of Veterinary Parasitology, v. 23, p. 150-156, 2014. HAJEK, A.E.; ST. LEGER, R.J. Interactions between fungal pathogens and insect hosts. Annual Review of Entomolology, v. 39, p. 293-322, 1994. HEMMATI, F.; PELL, J.K.; MCCARTNEY, H.A.; DEADMAN, M.L. Airborne concentrations of conidia of Erynia neoaphidis above cereal fields. Mycological Research, v. 105: p. 485?489, 2001. HORN, S. C.; ARTECHE, C. C. P. Situa??o parasit?ria da pecu?ria no Brasil. A Hora Veterin?ria, v. 4, p. 12-32, 1985. HUANG, B. F.; FENG, M. G. Comparative tolerances of various Beauveria bassiana isolates to UV-B irradiation with a descriptio of a modeling method to assess lethal dose. Mycopathologia. v. 168, p. 145-152, 2009. INGLIS, G. D.; GOETTEL, M. S.; TARIQ, M. B.; STRASSER, H. Fungi as Biological Control Agents. CAB International, Wallingford, pp. 23?69, 2001. JEFFS, L.B.; XAVIER, I.J.; MATAI, R.E.; KHACHATOURIANS, G.G. Relationships between fungal spore morphologiesand surface properties for entomopathogenic members of the genera Beauveria, Metarhizium, Paecilomyces, Tolypocladium e Verticillium. Canadian journal of Microbiology, v. 45, p. 936-948, 1999. JONSSON N. The productivity effects of cattle tick (Boophilus microplus) infestation on cattle, with particular reference to Bos indicus cattle and their crosses. Veterinary Parasitology, v. 137, p. 1-10, 2006. KAAYA, G. P.; MWANGI, E. N.; OUNA, E. A. Prospects for biological control of livestock ticks, Rhipicephalus appendiculatus and Amblyomma variegatum, using the entomogenous fungi Beauveria bassiana and Metarhizium anisopliae. Journal of Invertebrate Pathology, v. 67, p. 15-20, 1996. KAAYA, G. P.; HASSAN, S. Entomogenous fungi as promising biopesticides for tick control. Experimental and Applied Acarology, v. 24, p. 913?926, 2000. KIRKLAND, B. H.; WESTWOOD, G. S.; KEYHANI, N. O. Pathogenicity of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to ixodidae species Dermacentor variabilis, Rhipicephalus sanguineus, and Ixodes scapularis. Journal of Medical Entomology, v. 41, p. 705-711, 2004. KOCAN, K.M. Targeting ticks for control of selected haemoparasitic diseases of cattle. Veterinary Parasitology, v. 57, p. 121-151, 1995. LEEMON, D.M.; JONSSON, N.N. Laboratory studies on Australian isolates of Metarhizium anisopliae as a biopesticide for the cattle tick Boophilus microplus. J. Invertebrate Pathology, v. 97, p. 40?49, 2008a. LEEMON, D.M.; TURNER, L.B.; JONSSON, N.N. Pen studies on the control of cattle tick Rhipicephalus (Boophilus) microplus with Metarhizium anisopliae (Sorokin). Veterinary Parasitology, 156, 248?260, 2008b. LIU, H.; SKINNER, M.; BROWNBRIGDE, M.; PARKER, B.L. Characterization of Beauveria bassiana and Metarhizium anisopliae isolates for management of tarnished plant bug, Lygus lineolaris (Hemiptera: Miridae). Journal of Invertebrate Pathology, v.82, p.139- 147, 2003. LIU, B-L; TZENG, Y-M. Development and applications of destruxins: a review. Biothecnology Advances, v. 30, p. 1242-1254, 2012. LOMER, C.J.; BATEMAN, R.P.; JOHNSON, D.L.; LANGEWALD, J.; THOMAS, M. Biological control of locusts and grasshoppers. Annual Review of Entomology, v. 46, p. 667- 702, 2001. L?PEZ, E.; L?PEZ, G.; ORDUZ, S. Control de la garrapata Boophilus microplus com Metarhizium anisopliae, est?dios de laborat?rio y campo. Revista Colombiana de Entomologia, v. 35 (1), p. 42?46, 2009. LORD, J.C. From Metchnikoff to Monsanto and beyond: the path of microbial control. Journal of Invertebrate Pathology v. 89, p. 19?29, 2005. LUBECK, I.; ARRUDA, W.; SOUZA, B.K.; STANIS?UASKI, F.; CARLINI, C.R.; SCHANK, A.; VAINSTAIN, M.H. Evaluation of Metarhizium anisopliae strains as a potential biocontrol of the tick Rhipicephalus (Boophilus) microplus and the cotton stainer Dysdercus peruvianus. Fungal Ecology, v. 1, p. 78-88, 2008. LUZ, C.; TIGANO, M. S.; SILVA, I. G.; CORDEIRO, C. M. T.: ALJANABI, S. M. Selection of Beauveria bassiana and Metarhizuim anisopliae isolate to control Triatoma infestans. Mem?rias do Instituto Oswaldo Cruz, v. 93, p. 839-846, 1998. MANIANIA, N.K.; NCHU, F.; EKESI, S. Fungal pathogen for biocontrol of ticks. In: Ekesi, S., Maniania, N.K. (Eds.), Use of Entomopathogenic Fungi in Biological Pest Management. Research Signpost, Kerala, p. 275?294, 2007. MATA, R.F.F. Efeito de extratos aquosos de Cabralea canjerana subsp. polytricha (Adr. Juss) Penn. (Meliaceae) no controle biol?gico de Brevycorine brassicae (L.) (Hemiptera: Aphididae) e Ascia monuste orseis (Godart) (Lepidoptera: Pieridae). 2007. 66p. Disserta??o (Mestrado em Ecologia e Conserva??o de Recursos Naturais). Universidade Federal de Uberl?ndia, Uberl?ndia, 2007. MENT, D.; GINDIN, G.; GLAZER, I.; PERL, S.; ELAD, D.; SAMISH, M. The effect of temperature and relative humidity on the formation of Metarhizium anisopliae chlamydospores in tick eggs. Fungal Biology, v. 114, p. 49-56, 2010. MONTEIRO, A.C.; FIORIN, A.C.; CORREIA, A.C.B. Pathogenicity of isolates of Metarhizium anisopliae (Mestsch.) Sorokin towards the cattle tick Boophilus microplus (Can.) (Acari: Ixodidae) under laboratory conditions. Revista de Microbiologia, v. 29, p. 109-112, 1998. MOON, Y-S.; DONZELLI, B.G.G.; KRASNOFF, S.B.; McLANE, H.; GRIGGS, M.H.; COOKE, P.; VANDENBERG, J.D.; GIBSON, D.M.; CHURCHILL, A.C.L. Agrobacterium- mediated disruption of a nonribosomal peptide synthetase gene in the invertebrate pathogen Metarhizium anisopliae reveals a peptide spore factor. Applied and Environmental Microbiology, v. 74, p. 4366-4380, 2008. MURREL, A.; BARKER, S. C. Synonymy of Boophilus Crutice, 1891 with Rhipicephalus Koch, 1844 (Acari: Ixodidae). Systems Parasitology, v. 56, p. 169-172, 2003. OAKESHOTT, J.G.; HOME, I.; SUTHERLAND, T.D.; RUSSEL, R.J. The genomics of insecticide resistance. Genome Biology. v. 4: p. 202, 2003. OJEDA-CHI, M. M.; RODRIGUEZ-VIVAS, R. I.; GALINDO-VELASCO, E.; LEZAMA-GUTI?RRREZ, R. Laboratory and field evaluation of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) for the control of Rhipicephalus microplus (Acari: Ixodidae) in the Mexican tropics. Veterinary Parasitology, v. 170, p. 348?354, 2010. OLIVEIRA, G.P.; ALENCAR, M.M. Resist?ncia de bovinos de seis graus de sangue Holand?s-Guzer? ao carrapato (Boophilus microplus) e ao berne (D. hominis). Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, v. 42, p. 127-35, 1990. ONOFRE, S.B.; MINIUIK, C.M.; de BARROS, N.M.; AZEVEDO, J.L. Pathogenicity of four strains of entomopathogenic fungi against the bovine tick Boophilus microplus. American Journal of Veterinary Research, v. 62, p. 1478?1480, 2001. PEDRAS, M.S.C.; ZAHARIA, L.I.; WARD, D.E. The destruxins: synthesis, biosynthesis, biotransformation, and biological activity. Phytochemistry, v. 59, p. 579596, 2002. PARRA, J. R.; BOTELHO, P. S. M.; BENTO, J. M; FERREIRA, B. S. Controle Biol?gico no Brasil: parasit?ides e predadores. Editora Manole, S?o Paulo, 2002. 609p. PERINOTTO, W. M. S.; ANGELO, I. C.; GOLO, P. S.; QUINELATO, S. B.; CAMARGO, M. G.; SA, F. A.; BITTENCOURT, V. R. E. P. Susceptibility of different populations of ticks to entomopathogenic fungi. Experimental Parasitology, v. 130, p. 257- 260, 2012. PERINOTTO, W. M. S.; G?LO, P. S.; COUTINHO-RODRIGUES, C. J. B.; S?, F. A.; SANTI, L.; BEYS-DA-SILVA, W. O.; JUNGES, A.; VAINSTEIN, M. H.; SCHRANK, A.; SALLES, C. M. C.; BITTENCOURT, V. R. E. P. Enzymatic activities and effects of mycovirus infection on the virulence of Metarhizium anisopliae in Rhipicephalus microplus. Veterinary Parasitology, v. 203, p. 189-196, 2014. POLAR, P.; KAIRO, M.T.; MOORE, D.; PEGRAM, R.; JOHN, A.S. Comparison of water, oils and emulsifiable adjuvant oils as formulating agents for Metarhizium anisopliae for use in control of Boophilus microplus. Mycopathologia 160, 151?157, 2005. POWELL, R.T.; REID, T.J. Project tick control. Queensland Agricultural Journal, v. 108, p. 279-300, 1982. QUINELATO, S.; GOLO, P. S.; PERINOTTO, W. M. S.; S?, F. A.; CAMARGO, M. G.; ANGELO, I. C.; MORAES, A. M. L.; BITTENCOURT, V. R. E. P. Virulence potential of Metarhizium anisopliae s.l. isolates on Rhipicephalus (Boophilus) microplus larvae. Veterinary Parasitology, v. 190, p. 556?565, 2012. REBELO, R.M.; VASCONCELOS, R.A.; BUYS, B.D.M.C.; REZENDE, J.A.; MORAES, K.O.C.; OLIVEIRA, R.P. Pesticides and Related Commercialized in Brazil in 2009. An Environmental Approach; IBAMA Press: Brasilia, Brazil, pp. 30?84, 2010. RIDDELL, R.W. Permanent stained mycological preparations obtained by slide culture. Mycologia, v. 42, p. 265-270, 1950. ROCHA, C.M.B.M. O carrapato dos bovinos. Boletim T?cnico: S?rie Extens?o da Universidade Federal de Lavras. Lavras, ano VI, n. 6, 1997. 27p. ROY, H.E.; PELL, J.K.; ALDERSON, P.G. Targeted dispersal of the aphid pathogenic fungus Erynia neoaphidis by the aphid predator Coccinella septempunctata. Biocontrol Science Technology 11:99?110, 2001. SAMISH, M.; GINSBERG, H.; GLAZER, I. Biological control of ticks. Parasitology. v. 129, p. 389-413, 2004. SAMSON, R. A.; EVANS, H. C. Two new Beauveria spp. from South America. Journal of Invertebrate Pathology, v. 39, p. 93-97, 1982. SCHRANK, A.; VAINSTEIN, M.H. Metarhizium anisopliae s.l. enzymes and toxins. Toxicon. v. 56, p. 1267-1274, 2010. SHAH, P.A.; PELL, J.K. Entomopathogenic fungi as biological control agents. Applied Microbiology Biotechnology v. 61, p. 413-423, 2003. SHAPIRO-ILAN, D.I.; FUXA, J.R.; LACEY, L.A.; ONSTAD, D.W.; KAYA, H.K. Definitions of pathogenicity and virulence in invertebrate pathology. Journal of Invertebrate Pathology. v. 88, p. 1-7, 2005. SILVA, A.M.; ALENCAR,M.M.; REGITANO, L.C.A.; OLIVEIRA, M.C.S. Infesta??o natural de f?meas bovinas de corte por ectoparasitas na Regi?o Sudeste do Brasil. Revista Brasileira de Zootecnia, v. 39, n. 7, p. 1477-1482, 2010. ST. LEGER, R.J.; MAY, B.; ALLEE, L.L.; FRANK, D.C.; STAPLES, R.C.; ROBERTS, D.W. Genetic differences in allozymes and in formation of infection structures among isolates of the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, v. 60, p. 89-101, 1992. ST LEGER, R.J.; JOSHI, L.; BIDOCHKA, M.J.; ROBERTS, D.W. Construction of an improved mycoinsecticide overexpressing a toxic protease. Proceedings of the National Academy of Sciences, v. 93, p. 6349-6354, 1996. THOMAS, M.B.; READ, A.F. Fungal bioinsecticide with a sting. Nature Biotechnology v. 25, p. 1367-1368, 2007. TULLOCH, M. The genus Metarhizium. Transactions of British Mycology Society, v. 66, p. 407-411, 1976. WASSWEMANN, M.; SELZER, P.; STEIDLE, J.L.M.; MACKENSTEDT, U. Biological control of Ixodes ricinus larvae and nymphs with Metarhizium anisopliae blastospores. Ticks and Tick-borne Diseases, 2016. WEBSTER, A.; RECK, J.; SANTI, L.; SOUZA, U.A.; AGNOL, B.D.; KLAFKE, G.M.; BEYS-DA-SILVA, W.O.; MARTINS, J.R.; SCHRANK, A. Integrated control of an acaricide-resistant strain of the cattletick Rhipicephalus microplus by applying Metarhizium anisopliae associated with cypermethrin and chlorpyriphosunder field conditions. Veterinary Parasitology 207, 302?308, 2015. WHARTHON, R.H.; UTECH, K.B.W.; TURNER, H.G. Resistance to the cattle tick, B. microplus, in a herd of Australian Illawarra Shorthorn cattle: its assessment and herdability. Australian JournaL Agriculture Research, v. 21, p. 163-81, 1970. WHARTON, R. H. Ticks with special emphasis on Boophilus microplus. In: PAL, R.; WHARTON, R. H. Control of arthropods of medical and veterinary importance. New York, Plenum Publishing, 1974, p. 36-52. ZHIOUA, E.; M. BROWNING, P. W.; JOHNSON, H. S.; GINSBERG, AND R. A. LEBRUN. Pathogenicity of the entomopathogenic fungus Metarhizium anisopliae (Deuteromycetes) to Ixodes scapularis (Acari: Ixodidae). Journal of Parasitology. v. 83, p. 815-818, 1997. ZIMMERMANN, G. The entornopathogenic fungus Metarhizium anisopliae and its potential as a biocontrol agent. Pesticide. Science. v. 37, p. 375?379, 1993. |
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