A new compound, clerodane diterpenoid, CD, was isolated from the bark of Polyalthia longifolia var. pendula. . In previously study, CD was proved to induce cell apoptosis. We analysis differentially expressed proteins of colorectal cancer cells under drug CD treatment by 2-D electrophoresis and find drug response proteins, e.g. Hsp10, Profilin-1, Peroxindoxin-1. The expression change of protein had been further confirmed by RT-PCR and western blotting. It is interesting to reveal the role of these proteins in the colorectal cancinogenesis and anti-tumor drug response. In most kind of cancers, Hsp10 is often overexpress as indicated in many reports. Under CD treatment, Hsp10 is down-regulation in our experiment. The major aim of this study is to investigate the effect of Hsp10 on colorectal cancer cell lines SW480, and also investigate the relation of Hsp10 to drug treatment. Here,we overexpress Hsp10 in SW480. MTT assay, and flow cytometric analysis are utilized to investigate the effect of Hsp10 and CD cytotoxicity when overrxpress Hsp10 in SW480.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0819110-175708 |
| Date | 19 August 2010 |
| Creators | Chen, Wei-cheng |
| Contributors | Kuang-Hung Cheng, Hsueh-Wei Chang, Hung-Wen Huang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0819110-175708 |
| Rights | not_available, Copyright information available at source archive |
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