Spiral-artery (SA) remodeling is a fundamental process during pregnancy that involves the
action of cells of the initial vessel, such as vascular smooth-muscle cells (VSMCs) and endothelial
cells, but also maternal immune cells and fetal extravillous trophoblast cells (EVTs). Mast cells
(MCs), and specifically chymase-expressing cells, have been identified as key to a sufficient SA remodeling process in vivo. However, the mechanisms are still unclear. The purpose of this study
is to evaluate the effects of the MC line HMC-1 and recombinant human chymase (rhuCMA1)
on human primary uterine vascular smooth-muscle cells (HUtSMCs), a human trophoblast cell
line (HTR8/SV-neo), and human umbilical-vein endothelial cells (HUVEC) in vitro. Both HMC-1
and rhuCMA1 stimulated migration, proliferation, and changed protein expression in HUtSMCs.
HMC-1 increased proliferation, migration, and changed gene expression of HTR8/SVneo cells, while
rhuCMA treatment led to increased migration and decreased expression of tissue inhibitors of matrix
metalloproteinases. Additionally, rhuCMA1 enhanced endothelial-cell-tube formation. Collectively,
we identified possible mechanisms by which MCs/rhuCMA1 promote SA remodeling. Our findings
are relevant to the understanding of this crucial step in pregnancy and thus of the dysregulated
pathways that can lead to pregnancy complications such as fetal growth restriction and preeclampsia.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:85988 |
| Date | 13 June 2023 |
| Creators | Zhang, Ningjuan, Schumacher, Anne, Fink, Beate, Bauer, Mario, Zenclussen, Ana Claudia, Meyer, Nicole |
| Publisher | MDPI |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1158 |
Page generated in 0.0085 seconds