Auxin can induce adventitious rooting. Synthetic auxin, indole-3-butyric acid (IBA), effectively promoted the rooting in Cinnamomum kanehirae. In Cinnamomum kanehirae, there are high (H) and low (L) rooting cultivar. The peroxidase (POX) activity significantly decreased in the IBA-treated tissues as compared with the control. The inhibition on POX activity may lead to the redifferentiation processes induced by IBA. In this investigation, we cloned POX cDNA from the young roots. Degenerate primers were designed from the conservative regions of other published POXs to amplify the expectant DNA fragment. We found that the H and L line have similar genes (>99%). The Full-length cDNA of the POX genes were cloned by the method of 5'and 3' Rapid Amplification of cDNA Ends (RACE). The deduced amino acid were compared with the previously reported POX and showed between 40% and 70% identity with other plant POXs. Further studies on the promoter elements of POX in High-rooting cultivar and Low-rooting line show that some elements are related to auxin response, lignification, pathogen invasion and stress response.
The regulatory elements of the POX gene in High-rooting line contain sugar repression responsiveness (SRS) elements that might repress the expression of POX gene, causing the lower POX activity.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0526109-130638 |
| Date | 26 May 2009 |
| Creators | Li, Ming-wei |
| Contributors | You-Chung Chiang, Ching-Huang Liu, Chi-Chu Tsai, Chang-Hung Chou |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0526109-130638 |
| Rights | restricted, Copyright information available at source archive |
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