MSc (Med), Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of the Witwaterstrand / Emergence and spread of resistance to ciprofloxacin among Neisseria
gonorrhoeae strains has reduced the options of effective treatment for
gonococcal infections and has become a concern worldwide. Up until 2008,
ciprofloxacin was recommended first-line therapy for treatment of presumptive N.
gonorrhoeae infections in South Africa. At the time this MSc project was
conceived, ciprofloxacin was still used as first-line therapy for presumptive
gonococcal infections.
A real-time polymerase chain reaction (PCR) assay was used to detect
ciprofloxacin-resistant N. gonorrhoeae in DNA extracted from non-invasive urine
samples collected as part of the national microbiological surveillance (NMS)
programme during 2006-2007. The molecular epidemiology of ciprofloxacinresistant
Neisseria gonorrhoeae was investigated by sequencing the quinolone
resistance determining regions (QRDR) of the gyrA and parC genes of N.
gonorrhoeae and performing N. gonorrhoeae multi-antigen sequence typing (NGMAST).
As part of the NMS program for sexually transmitted infections (STIs) urine and
urethral swabs were collected from men presenting with urethral discharge at
primary health care clinics in Johannesburg (Gauteng), Cape Town (Western
Cape) and Kimberley (Northern Cape). Urine samples and cultured N.
gonorrhoeae isolates from 2006-2007 were stored at -700C and available for this
study. Gonococci, previously isolated from urethral swabs, were subcultured
directly onto New York City media. Isolate identity was re-confirmed by typical
colony morphology and biochemical tests. Urine samples from Johannesburg
were tested in order to develop the real-time PCR protocol. Subsequently,
paired urethral swab DNA and N. gonorrhoeae cultures were tested from NMS
patients recruited in Kimberley and Cape Town. Where possible, the PCR assay
results were compared with paired antibiotic susceptibility data for ciprofloxacin.
Quinolone resistance determining regions (QRDR) for gyrA and parC were screened for known point mutations associated with resistance to ciprofloxacin.
Detection of mutations by the real-time PCR assay generally agreed with the
phenotype of either decreased susceptibility or resistance to ciprofloxacin. All
ciprofloxacin resistant gonococcal isolates had the same gyrA and parC
mutations, which initially suggested that quinolone resistant N. gonorrhoeae
(QRNG) in Kimberley, Cape Town and Johannesburg, may be attributed to the
spread of a single clone. The use of a more discriminatory typing scheme,
Neisseria gonorrhoeae Multi-Antigen Sequence Typing (NG-MAST) genotyping,
revealed that ciprofloxacin resistant gonococcal isolates in Johannesburg and
Cape Town were heterogeneous, with sequence type (ST) 217 being most
prevalent in both cities (5/16, Johannesburg; 7/11, Cape Town). In contrast, all
eight QRNG isolates from Kimberley were typed as ST 533.
The use of molecular methods allowed ciprofloxacin antimicrobial susceptibility
determination by PCR in non-invasive specimens. This is useful in situations
where bacterial cultures are unavailable or die before antimicrobial susceptibility
testing can be performed. Molecular assays to detect ciprofloxacin resistance
may guide physicians as to the most ideal antimicrobial combinations for
individual patient treatment.
As a result of emerging widespread resistance gonococci to ciprofloxacin, in
2008, the Department of Health recommended that ciprofloxacin be removed as
a first line therapy in the South African national sexually transmitted infections
treatment guidelines for treatment of urethritis, cervicitis and their complications.
Although ciprofloxacin is no longer used as a first-line therapy to treat
gonorrhoea within our country, it may still be used in cases of severe penicillin
allergy or as part of multi-drug therapy for gonococcal infections in the future.
The ability to detect ciprofloxacin resistance by real-time PCR will be a useful
technique in such situations.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:wits/oai:wiredspace.wits.ac.za:10539/9187 |
| Date | 22 March 2011 |
| Creators | Magooa, Mahlape Precious |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | application/pdf |
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