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Avalia??o histol?gica do reparo em enxertos de derme acelular, estimulado por laser n?o ablativo

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Previous issue date: 2012-03-08 / This study aimed to evaluate the integration of an acellular dermal matrix graft (AlloDerm?; LifeCell Corp., The Woodlands, TX) at the implantation site and to compare integration rates between groups receiving and not receiving nonablative laser treatment by the quantification of fibroblasts within the graft and assessment of the healing process and inflammatory response around the graft. Standard 5-mm2 AlloDerm? sheets were implanted into the subcutaneous tissue in the calvaria of 32 Wistar rats (Rattus Norvegicus). Laser therapy was given at a dose of 4 J/cm2 per session immediately after surgical intervention, followed by 3 additional applications at 48-h intervals. The animals were killed 1, 3, 7, and 14 days after surgery. The specimens were stained with hematoxylin-eosin (HE). Analysis of the slides showed a significantly higher amount of edema in the control group at all observation time points, compared to the laser-irradiated group. Absence of neutrophilic inflammatory infiltrate was observed in the laser-irradiated group at all time points, but a significant amount of this infiltrate was found in the control group at 3 of the 4 observation time points. Lymphocytic infiltration was evident as early as the first observation time point in laser-irradiated animals, whereas in controls this type of infiltration was observed only after the second time point. In addition, the control group showed significantly greater revascularization than the laser-irradiated group. There was no significant difference between groups in the quantity of fibroblasts within the graft. These findings suggest that additional nonablative laser therapy is effective in the healing and integration of acellular dermal grafts. / Este trabalho tem por objetivo avaliar a integra??o do enxerto de derme acelular Alloderm? (LifeCell Corp., The Woodlands, TX) em s?tios de implanta??o e comparar tal integra??o entre grupos que receberam incid?ncia de laser n?o ablativo e grupos que n?o receberam essa incid?ncia, por meio de an?lise da quantidade de fibroblastos no interior do enxerto e de avalia??o do processo cicatricial e da reposta inflamat?ria do organismo em torno do mesmo. Foram inseridos no tecido subcut?neo da regi?o calvaria de 32 ratos (Rattus Norvegicus, Wistar) fragmentos padr?es de 5mm2 de Alloderm?. Aplicou-se laserterapia de 4J/cm2 por sess?o iniciando imediatamente ap?s a interven??o cir?rgica e fazendo mais tr?s incid?ncias com intervalos de 48 horas. As mortes dos animais ocorreram no primeiro, terceiro, s?timo e d?cimo quarto dias de p?s operat?rio e as pe?as foram coradas com Hematoxilina-Eosina (H.E.). Na an?lise das l?minas foi observada, presen?a significativamente maior de edema no grupo controle, em todos os per?odos de observa??o, em compara??o ao grupo irradiado com laser. Observou-se tamb?m aus?ncia de infiltrado inflamat?rio neutrof?tico no grupo irradiado com laser em todos os per?odos estudados, estando o mesmo infiltrado presente significativamente no grupo controle em tr?s dos quatro per?odos estudados. Observou-se infiltrado linfoc?tico desde o primeiro per?odo de verifica??o no grupo irradiado, sendo que no grupo controle este tipo de infiltrado come?ou a ser observado no segundo per?odo de observa??o. Al?m disso, observou-se ainda, uma vasculariza??o bem mais intensa no grupo controle quando comparado ao irradiado. N?o se verificou diferen?a significativa entre os grupos na quantidade de fibroblastos no interior do enxerto. Os resultados encontrados sugerem que a terapia adicional com laser n?o ablativo ? efetiva no processo cicatricial e na integra??o do enxerto de derme acelular.

Identiferoai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/1159
Date08 March 2012
CreatorsSilveira, Vinicius Salim
ContributorsOliveira, Mar?lia Gerhardt de
PublisherPontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Odontologia, PUCRS, BR, Faculdade de Odontologia
Source SetsIBICT Brazilian ETDs
LanguagePortuguese
Detected LanguageEnglish
Typeinfo:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis
Formatapplication/pdf
Sourcereponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS
Rightsinfo:eu-repo/semantics/openAccess
Relation-8096554818733665164, 500, 600, 4673435736271820140

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