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Previous issue date: 2013-08-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / This report describes the fabrication of a minimally instrumented polyestertoner
device (PTD) to perform by the first time, clinical diagnostics with
colorimetric detection and microfluidic transport by capillary action. A
conventional digital camera, a cellphone camera, an optical microscope, and
a scanner mode from a multifunction printer were evaluated for using as
colorimetric detector. In order to study the analytical performance it was used
a platform containing nine detection zones directly printed on a polyester film.
It has been observed that all electronic devices studied could be applied as
colorimetric detector. However, the scanner exhibited the best performance
on the control of two problems often observed in the images acquisition,
which are related to the ambient light and focus. Due to the best results, the
analytical feasibility of the scanner was evaluated for the determination of
Fe2+ in drugs samples by using the 1,10-phenanthroline method. The data
were compared to results achieved by a reference spectrophotometric
method. Based on a statistical analysis, it was not been found significance
differences (P=0,05). Afterwards, the scanner was chosen to monitor the
colorimetric response of clinical assays regarded to the detection of glucose,
total protein, cholesterol, and triglycerides on a PTD. The proposed device
exhibited good inter and intra-device reproducibility (relative standard
deviation values lower than 6%). A semi-quantitative analysis of glucose,
total protein, and cholesterol in an artificial serum sample provided results
similar to the data provide by the supplier. The shelf life of PTDs was
evaluated in a period of five days, using the glucose assay at three different
temperature: 10 ºC, 25 ºC e 40 ºC. The shelf life was estimated to be c.a.
three days, taking into account that in this period there was a lost of 50% in
the mean color intensity. Lastly, it was observed that the addition of a
disaccharide (trehalose) ensures the enzymatic stability at the different
temperatures during five consecutive days. The cost of a PTD has been
estimated to be ca. R$ 0.40 taking into account the fabrication process. / O presente trabalho descreve a construção de dispositivos em
poliéster-toner (DPTs) minimamente instrumentados para conduzir, pela
primeira vez, diagnósticos clínicos utilizando detecção colorimétrica e
transporte microfluídico com ação capilar. Uma câmera digital convencional,
uma câmera de celular, um microscópio óptico e o modo scanner de uma
impressora multifuncional foram avaliados como detectores colorimétricos.
No estudo do desempenho analítico utilizou-se uma plataforma com nove
zonas de detecção impressas diretamente em uma folha de poliéster. Todos
os dispositivos eletrônicos avaliados podem ser aplicados como detector
colorimétrico. Entretanto, o scanner demonstrou facilidade no controle dos
dois problemas que dificultam a aquisição das imagens: quantidade de luz
do ambiente e foco. Frente a essa facilidade, realizou-se um estudo
comparando o desempenho analítico do scanner em relação a um
espectrofotômetro, utilizando a determinação de Fe2+ pelo método da 1,10-
fenantrolina. Cálculos estatísticos foram realizados e não foram observadas
diferenças significativas entre o desempenho analítico dos equipamentos
(P=0,05). Após, a avaliação do desempenho analítico, o scanner foi
escolhido para monitorar a resposta colorimétrica dos ensaios clínicos de
glicose, proteínas totais, colesterol e triglicerídeos, usando os DPTs. O
dispositivo proposto apresentou boa repetibilidade inter e intra-dispositivos
com desvio padrão relativo abaixo de 6%. Uma análise semi-quantitativa
para glicose, proteínas totais e colesterol foi conduzida utilizando uma
amostra de soro humano artificial. Os níveis de concentração encontrados
estavam de acordo com os dados fornecidos pelo fabricante. O tempo de
meia-vida dos DPTs foi avaliado ao longo de cinco dias, utilizando o ensaio
para glicose em diferentes temperaturas: 10 ºC, 25 ºC e 40 ºC. A meia-vida
dos DPTs foi estimado em três dias, considerando que nesse período houve
um decréscimo de 50% na intensidade média de pixels, da cor desenvolvida.
Observou-se que a adição de um dissacarídeo (trealose) assegurou a
estabilidade enzimática em diferentes temperaturas ao longo de cinco dias
consecutivos. O custo total de um DPT, considerando o processo de
fabricação, foi estimado em R$ 0,40.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.bc.ufg.br:tede/3165 |
| Date | 28 August 2013 |
| Creators | Souza, Fabrício Ribeiro de |
| Contributors | Coltro, Wendell Karlos Tomazelli, Coltro, Wendell Karlos Tomazelli, Piccin, Evandro, Oliveira, Anselmo Elcana de |
| Publisher | Universidade Federal de Goiás, Programa de Pós-graduação em Química (IQ), UFG, Brasil, Instituto de Química - IQ (RG) |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | Portuguese |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFG, instname:Universidade Federal de Goiás, instacron:UFG |
| Rights | http://creativecommons.org/licenses/by-nc-nd/4.0/, info:eu-repo/semantics/openAccess |
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