by Chiu Pui Yu. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 86-118). / Abstracts in English and Chinese. / Title Page --- p.i / Abstract --- p.ii-iv / Acknowledgement --- p.v / Table of Contents --- p.vi-viii / List of Tables --- p.ix / List of Figures --- p.x-xii / List of Abbreviations --- p.xiii / Chapter Section I: --- Introduction / Chapter Chapter 1: --- Overview --- p.1-2 / Chapter Chapter 2: --- Teratogenic Effects of Hyperglycemia / Chapter 2.1 --- What is Hyperglycemia --- p.3 / Chapter 2.2 --- Teratogenic Effects of Hyperglycemia --- p.4-6 / Chapter 2.2.1 --- Human Studies / Chapter 2.2.2 --- Animal Studies / Chapter 2.3 --- Timetables for Embryogenesis: Rats versus Humans --- p.7 / Chapter 2.4 --- Mechanisms of Hyperglycemia Induced Teratogenesis --- p.8-12 / Chapter 2.4.1 --- What are Free Radicals? / Chapter 2.4.2 --- Major Free Radical Species Involvedin Hyperglycemic Teratogenesis / Chapter 2.4.3 --- Molecular Damage Induced by Reactive Oxygen Species / Chapter 2.4.4 --- Supporting Evidence of Reactive Oxygen Species Causing Anomalies / Chapter 2.4.5. --- Hyperglycemia and Formation of Free Radicals / Chapter Chapter 3: --- Caffeine as Teratogen and Antioxidant / Chapter 3.1 --- Popularity of Caffeine --- p.13 / Chapter 3.2 --- Basic Metabolism of Caffeine --- p.14 / Chapter 3.3 --- Biological Actions of Caffeine --- p.15 / Chapter 3.4 --- Teratogenicity of Caffeine --- p.16-20 / Chapter 3.4.1 --- Animal Studies / Chapter 3.4.1.1 --- Teratogenic Effects of Caffeine in Animals / Chapter 3.4.1.2 --- Teratogenic Dose of Caffeine / Chapter 3.4.1.3 --- Interspecies Sensitivity / Chapter 3.4.2 --- Human Studies / Chapter 3.5 --- Possible Mechanisms for the Teratogenic Actions of Caffeine --- p.21 / Chapter 3.6 --- Caffeine as an Antioxidant --- p.22 / Chapter 3.7 --- Combined Effects of Caffeine with Other Substances --- p.23 / Chapter Chapter 4: --- Combined Effects of Hyperglycemia and Caffeine on Early Embryogenesis- A Question to be Answered / Chapter 4.1 --- Possible Links between Hyperglycemia and Caffeine --- p.24 / Chapter 4.2 --- Objectives of the Present Study --- p.25 / Chapter 4.3 --- Hypothesis --- p.26 / Chapter Section II: --- Research Designs and Methods / Chapter Chapter 5: --- Materials and Methods / Chapter 5.1 --- Licenses --- p.27 / Chapter 5.2 --- Overall Study Design --- p.28-40 / Chapter 5.2.1 --- Whole Embryo Culture Model / Chapter 5.2.1.1 --- Animals / Chapter 5.2.1.2 --- Explantation of Embryos and Serum Collection / Chapter 5.2.1.3 --- Preparation of Serum / Chapter 5.2.1.4 --- Culture Media / Chapter 5.2.1.5 --- Embryo Culture / Chapter 5.2.2 --- Experimental Groups / Chapter 5.2.3 --- Morphological Assessment / Chapter 5.2.4 --- Quantitation of Oxidative Stress / Chapter 5.2.5 --- Protein Assay / Chapter 5.3 --- Statistical Evaluation --- p.41 / Chapter Chapter 6: --- Laboratory Considerations / Chapter 6.1 --- Whole Embryo Culture Model --- p.42-43 / Chapter 6.1.1 --- Subjects / Chapter 6.1.2 --- Time Mating / Chapter 6.1.3 --- Culture Medium / Chapter 6.1.4 --- Gas Phase and Rotating Bottle Culture Method / Chapter 6.2 --- Quantification of Oxidative Stress --- p.47-49 / Chapter 6.2.1 --- 8-Isoprostaglandins F2a as a Marker / Chapter 6.2.2 --- Assay for 8-Isoprostaglandins F2a / Chapter 6.2.2.1 --- Enzyme Immunoassay versus Gas Chromatography/ Mass Spectrometry / Chapter Section III: --- Results / Chapter Chapter 7: --- Results / Chapter 7.1 --- Justifications of Methods of Statistical Analysis --- p.50 / Chapter 7.2 --- Effects of Hyperglycemia on Early Embryogenesis --- p.51-56 / Chapter 7.2.1 --- Effects of Hyperglycemia on Morphological Development / Chapter 7.2.2 --- Effects of Hyperglycemia on Production of 8-isoprostaglandins F2a / Chapter 7.2.3 --- Effects of Hyperglycemia on Total Protein Content / Chapter 7.3 --- Effects of Caffeine on Early Embryogenesis --- p.57-61 / Chapter 7.3.1 --- Effects of Caffeine on Morphological Development / Chapter 7.3.2 --- Effects of Caffeine on Total Protein Content / Chapter 7.4 --- Combined Effects of Hyperglycemia and Caffeine on Early Embryogenesis --- p.62-66 / Chapter 7.4.1 --- Combined Effects of Hyperglycemia and Caffeine on Morphological Development / Chapter 7.4.2 --- Combined Effects of Hyperglycemia and Caffeine on Production of 8-isoprostaglandins F2a / Chapter 7.4.3 --- Combined Effects of Hyperglycemia and Caffeine on Total Protein Content / Chapter Section IV: --- Discussion and Conclusions / Chapter Chapter 8: --- Discussion --- p.67-83 / Chapter Chapter 9: --- Conclusions and Future Directions --- p.84 / Appendices --- p.85 / References --- p.86-118
| Identifer | oai:union.ndltd.org:cuhk.edu.hk/oai:cuhk-dr:cuhk_323656 |
| Date | January 2001 |
| Contributors | Chiu, Pui Yu., Chinese University of Hong Kong Graduate School. Division of Obstetrics and Gynaecology. |
| Source Sets | The Chinese University of Hong Kong |
| Language | English, Chinese |
| Detected Language | English |
| Type | Text, bibliography |
| Format | print, xiii, 117 leaves : ill. ; 30 cm. |
| Rights | Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
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