Corneal dystrophies are commonly referred to as an congenital condition. Surgical complications are usually worse then the primary dysfunction and patients need to go through large surgical process. Only 25 % of the patients reach driving licence vision after a surgery. The aim of this study was to investigate if the application of human embryonic stem cells (hESc) could replace epithelial cells of the human cornea. Corneal markers such as cytokeratins CK3, CK15, CK19 and Pax - 6 were analysed by immunohistochemistry. HES - Cellect was used as indication of stem cell potential of the transplanted cells. hESc transplanted onto the cornea could be seen to attach and expand dominantly towards Bowman’s membrane. Human embryonic stem cells in culture were relatively positive for markers, contradictionally stem cells in the epithelial trails lost their stem differentiation potential and appeared to be negative for all markers used in these trails. Optimization of stem cells differentiation into epithelial which may in the future may gives us the ability to perform clinical applications with successful outcome.
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:sh-3672 |
| Date | January 2010 |
| Creators | Gurajada, Deepthi |
| Publisher | Södertörns högskola, Institutionen för livsvetenskaper |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
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